{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T21:44:45Z","timestamp":1648503885260},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1992,4,1]]},"abstract":"<jats:p> Intracellular microelectrode techniques were used to characterize basolateral membrane K+ conductances in isolated Necturus antral mucosa. Exposure of tissues to progressively higher levels of serosal K+ (4, 20, 40, or 60 mM) resulted in progressively greater depolarizations of basolateral membrane potentials and decreases in membrane resistance, consistent with the presence of a significant K+ conductance. Ba2+ (2 mM) partially blocks these conductances. Exposure of tissues to increased levels of serosal Ca2+ (from 1.8 to 6.8 mM) elicited significant hyperpolarization of basolateral potentials and decreases in basolateral resistance. These effects are also elicited by Sr2+ (5 mM), but not by Mg2+ (5 mM). Ba2+ (5 mM) elicits complex and time-dependent effects, but transiently elicits an effect similar to high Ca2+. Ion substitutions in the serosal perfusate suggest that the Ca(2+)-induced effects are due to enhancement of basolateral K+ conductances. Further work is necessary to identify the processes that mediate this increase in basolateral K+ conductance and to evaluate the physiological significance of this change in membrane permeability to K+. <\/jats:p>","DOI":"10.1152\/ajpgi.1992.262.4.g651","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T21:46:13Z","timestamp":1514065573000},"page":"G651-G659","source":"Crossref","is-referenced-by-count":0,"title":["Basolateral K+ conductances in surface epithelium of Necturus antrum: effects of Ca2+ and divalent cations"],"prefix":"10.1152","volume":"262","author":[{"given":"D. I.","family":"Soybel","sequence":"first","affiliation":[{"name":"Department of Surgery, Yale University School of Medicine, New Haven,Connecticut 06510."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. W.","family":"Ashley","sequence":"additional","affiliation":[{"name":"Department of Surgery, Yale University School of Medicine, New Haven,Connecticut 06510."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Y.","family":"Cheung","sequence":"additional","affiliation":[{"name":"Department of Surgery, Yale University School of Medicine, New Haven,Connecticut 06510."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1992.262.4.G651","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T18:45:31Z","timestamp":1567968331000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1992.262.4.G651"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,4,1]]}},"alternative-id":["10.1152\/ajpgi.1992.262.4.G651"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1992.262.4.g651","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,4,1]]}}}