{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T15:40:22Z","timestamp":1649086822218},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1992,6,1]]},"abstract":"<jats:p> Recent studies from our laboratory have demonstrated that the in vitro addition of 1,25-dihydroxycholecalciferol [1,25(OH)2D3] rapidly (seconds to minutes) stimulated membrane phosphoinositide turnover, translocated protein kinase C from the cytosolic to particulate fraction, increased cytosolic calcium ([Ca2+]i), and decreased cytoplasmic pH (pHi) via inhibition of Na(+)-H+ exchange in rat colonic epithelium of dietary vitamin D-sufficient rats and in Caco-2 cells. In contrast to these prior findings, in the present experiments, 1,25(OH)2D3 failed to elicit any of these colonic biochemical responses in vitamin D-deficient animals. Bethanechol chloride also failed to alter this signal transduction pathway, [Ca2+]i, or pHi. In vivo administration of this hormone for 5-7 days, moreover, to vitamin D-deficient animals restored the rapid biochemical effects of in vitro 1,25(OH)2D3 and bethanechol chloride. These studies, therefore, indicate that alterations in the vitamin D status of rats modulate the action of 1,25(OH)2D3 and other agents on the colonic phosphoinositide signal transduction system and on [Ca2+]i, which, in turn, may influence important cellular processes in this organ such as Na(+)-H+ exchange. <\/jats:p>","DOI":"10.1152\/ajpgi.1992.262.6.g945","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T02:36:45Z","timestamp":1514083005000},"page":"G945-G953","source":"Crossref","is-referenced-by-count":0,"title":["Effect of vitamin D status on the rapid actions of 1,25-dihydroxycholecalciferol in rat colonic membranes"],"prefix":"10.1152","volume":"262","author":[{"given":"R. K.","family":"Wali","sequence":"first","affiliation":[{"name":"Department of Medicine, Pritzker School of Medicine, University ofChicago, Illinois 60637."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. L.","family":"Baum","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pritzker School of Medicine, University ofChicago, Illinois 60637."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. D.","family":"Sitrin","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pritzker School of Medicine, University ofChicago, Illinois 60637."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Bolt","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pritzker School of Medicine, University ofChicago, Illinois 60637."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. K.","family":"Dudeja","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pritzker School of Medicine, University ofChicago, Illinois 60637."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. A.","family":"Brasitus","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pritzker School of Medicine, University ofChicago, Illinois 60637."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1992.262.6.G945","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T22:42:31Z","timestamp":1567982551000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1992.262.6.G945"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1992,6,1]]}},"alternative-id":["10.1152\/ajpgi.1992.262.6.G945"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1992.262.6.g945","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,6,1]]}}}