{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T19:57:11Z","timestamp":1648929431599},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1992,10,1]]},"abstract":"<jats:p> Transmembrane movements of K+ and Cl- in salivary acinar cells are important in the formation of saliva, and may be affected by changes in intracellular pH (pHi). Exposure to NH4Cl increases pHi transiently, but NH4+ may have effects independent of pHi. To investigate how Cl- transport may be altered under these conditions, rat submandibular acini were exposed to NH4Cl, and transmembrane Cl- transport was studied with 36Cl-. NH4Cl increased intracellular Cl- in these cells. The initial phase of this increase was partially HCO(3-)-dependent and was inhibited by 1 mM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), while the sustained phase was inhibited by 0.1 mM bumetanide. NH4Cl also inhibited acetylcholine-induced Cl- efflux from tracer preloaded cells. Changes in pH did not always correlate in time or extent with those of Cl- transport. We conclude that 1) exposure to NH4Cl increases Cl-uptake primarily by a bumetanide-sensitive transport system that did not reach steady state during the experiment, 2) exposure to NH4Cl also stimulates Cl- uptake by a DIDS-sensitive mechanism, and 3) only the latter is pHi sensitive. <\/jats:p>","DOI":"10.1152\/ajpgi.1992.263.4.g558","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T22:47:58Z","timestamp":1514069278000},"page":"G558-G565","source":"Crossref","is-referenced-by-count":0,"title":["Effects of NH4Cl and dimethylamine on Cl- fluxes in resting and stimulated rat submandibular acinar cells"],"prefix":"10.1152","volume":"263","author":[{"given":"J. C.","family":"Seagrave","sequence":"first","affiliation":[{"name":"Center for Basic and Applied Medical Research, Lovelace MedicalFoundation, Albuquerque, New Mexico 87108."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Barker","sequence":"additional","affiliation":[{"name":"Center for Basic and Applied Medical Research, Lovelace MedicalFoundation, Albuquerque, New Mexico 87108."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Curry","sequence":"additional","affiliation":[{"name":"Center for Basic and Applied Medical Research, Lovelace MedicalFoundation, Albuquerque, New Mexico 87108."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Center for Basic and Applied Medical Research, Lovelace MedicalFoundation, Albuquerque, New Mexico 87108."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1992.263.4.G558","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T18:51:59Z","timestamp":1567968719000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1992.263.4.G558"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,10,1]]}},"alternative-id":["10.1152\/ajpgi.1992.263.4.G558"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1992.263.4.g558","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,10,1]]}}}