{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T20:40:40Z","timestamp":1649018440143},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1993,2,1]]},"abstract":"<jats:p> Effects of Ca2+ on adenosine 3',5'-cyclic monophosphate (cAMP)-mediated Cl- secretion were investigated in intact mucosa and isolated crypt cells of rabbit descending colon. Addition of 10 microM prostaglandin (PG)E2 or forskolin to tissues incubated in Ca(2+)-free medium increased the size of short-circuit current (Isc) and Cl- secretion as estimated by unidirectional 36Cl flux measurements (net flux = -2.31 +\/- 0.24 vs. -1.22 +\/- 0.10 mueq.h-1.cm-2, n = 4, P &lt; 0.001). Addition of 10 microM PGE2 to tissues incubated in 1.2 mM Ca2+ Ringer induced a 7-fold increase in mean cAMP level, whereas it produced an 11-fold increase in tissues exposed to Ca(2+)-free medium. Membrane preparations from whole mucosa incubated in Ca(2+)-free medium displayed a cyclic nucleotide phosphodiesterase activity significantly lower than controls (18.76 +\/- 0.54 vs. 31.20 +\/- 0.39 pmol cAMP. mg protein-1.min-1, means +\/- SE, n = 4, P &lt; 0.001). Ca2+ removal also affected adenylate cyclase (AC) responsiveness to agonists; AC activity increased in controls by 54 and 226% after stimulation with 10 microM PGE2 and forskolin, respectively, but it increased more (77 and 325%, respectively) after incubation in Ca(2+)-free solutions.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/ajpgi.1993.264.2.g252","type":"journal-article","created":{"date-parts":[[2017,12,23]],"date-time":"2017-12-23T22:43:15Z","timestamp":1514068995000},"page":"G252-G260","source":"Crossref","is-referenced-by-count":2,"title":["Role of calcium in chloride secretion mediated by cAMP pathway activation in rabbit distal colon mucosa"],"prefix":"10.1152","volume":"264","author":[{"given":"V.","family":"Calderaro","sequence":"first","affiliation":[{"name":"Institute of Pharmacology and Toxicology, First Faculty of Medicine, University of Naples, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Chiosi","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, First Faculty of Medicine, University of Naples, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Greco","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, First Faculty of Medicine, University of Naples, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Spina","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, First Faculty of Medicine, University of Naples, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Giovane","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, First Faculty of Medicine, University of Naples, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Quagliuolo","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, First Faculty of Medicine, University of Naples, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Servillo","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, First Faculty of Medicine, University of Naples, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Balestrieri","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, First Faculty of Medicine, University of Naples, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Illiano","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology, First Faculty of Medicine, University of Naples, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1993.264.2.G252","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T18:50:47Z","timestamp":1567968647000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1993.264.2.G252"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,2,1]]}},"alternative-id":["10.1152\/ajpgi.1993.264.2.G252"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1993.264.2.g252","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,2,1]]}}}