{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,21]],"date-time":"2024-07-21T04:37:13Z","timestamp":1721536633026},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1993,4,1]]},"abstract":"<jats:p> The mammalian liver possesses the ability to regenerate to its original size after a 70% partial hepatectomy (PHx). The capacity of rat Kupffer cells (KC) isolated at specific intervals after PHx to produce interleukin (IL)-1, IL-6, and prostaglandin E2 (PGE2) in response to endotoxin [lipopolysaccharide (LPS)] stimulation was evaluated in standard RPMI 1640 (1,200 microM L-arginine) and arginine-depleted RPMI 1640 (&lt; 10 microM L-arginine) media. Because KC function in an environment in which high arginase activity results in negligible L-arginine levels, the 10 microM L-arginine RPMI 1640 was used to simulate the hepatic microenvironment. Regenerating liver KC 12-120 h after PHx responded to LPS with a significantly greater (P &lt; 0.05) production of IL-1 and IL-6 in standard RPMI 1640. This enhancement of regenerating liver KC to produce IL-1 and IL-6 was increased (P &lt; 0.05) by placing these same KC in 10 microM arginine RPMI 1640 culture media. During the same time period, regenerating liver KC produced significantly elevated (P &lt; 0.01) PGE2, again with greater differences in the low-arginine media. In vivo KC PGE2 blockade by indomethacin (5 mg\/kg) significantly (P &lt; 0.05) inhibited hepatic regeneration. When the cyclooxygenase inhibitor indomethacin (10 microM) was added to cultures, the production of PGE2 by KC was prevented, and in arginine-depleted cultures, IL-1 and IL-6 production was upregulated (P &lt; 0.05). We conclude that during hepatic regeneration, KC IL-1 and IL-6 production is elevated and is controlled in an autoregulatory fashion by elevated KC PGE2 production. <\/jats:p>","DOI":"10.1152\/ajpgi.1993.264.4.g601","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T03:15:56Z","timestamp":1514085356000},"page":"G601-G608","source":"Crossref","is-referenced-by-count":3,"title":["Prostaglandin E2 downregulates Kupffer cell production of IL-1 and IL-6 during hepatic regeneration"],"prefix":"10.1152","volume":"264","author":[{"given":"J. A.","family":"Goss","sequence":"first","affiliation":[{"name":"Department of Surgery, Washington University School of Medicine, St.Louis, Missouri 63110."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Mangino","sequence":"additional","affiliation":[{"name":"Department of Surgery, Washington University School of Medicine, St.Louis, Missouri 63110."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. P.","family":"Callery","sequence":"additional","affiliation":[{"name":"Department of Surgery, Washington University School of Medicine, St.Louis, Missouri 63110."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. W.","family":"Flye","sequence":"additional","affiliation":[{"name":"Department of Surgery, Washington University School of Medicine, St.Louis, Missouri 63110."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1993.264.4.G601","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T22:49:23Z","timestamp":1567982963000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1993.264.4.G601"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,4,1]]}},"alternative-id":["10.1152\/ajpgi.1993.264.4.G601"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1993.264.4.g601","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,4,1]]}}}