{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T01:46:04Z","timestamp":1648777564997},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1993,5,1]]},"abstract":"<jats:p> Conventional and ion-selective microelectrodes were used to characterize transport of Cl- across the basolateral cell membranes of gastric surface epithelium in isolated preparations of gastric antrum of Necturus. Conventional, voltage-sensing electrodes were used to evaluate changes in membrane potentials and resistances during removal of Cl- from the nutrient perfusate. Liquid ion exchanger Cl(-)-selective microelectrodes were constructed and validated to measure intracellular Cl- activity (aiCl). Our data indicate that 1) aiCl (range 12-25 mM) is close to that predicted if Cl- is distributed across the cell membranes by electrochemical equilibrium, 2) aiCl is not influenced by changes in luminal Cl- content but is susceptible to changes in nutrient Cl- content, 3) Cl- conductances cannot be detected in the basolateral membrane and changes in membrane potentials do not influence aiCl, and 4) Cl- accumulation across the basolateral membrane depends on Na+ and the level of [K+] in the nutrient solution. Inhibition of K(+)-dependent Cl- accumulation, in the absence of nutrient Na+ or in the presence of the inhibitor bumetanide, was demonstrated. These findings suggest that basolateral Na(+)-K(+)-Cl- cotransport is important in regulating cell Cl- levels in surface cells of the gastric antrum in Necturus. <\/jats:p>","DOI":"10.1152\/ajpgi.1993.264.5.g910","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T03:30:38Z","timestamp":1514086238000},"page":"G910-G920","source":"Crossref","is-referenced-by-count":1,"title":["Characteristics of basolateral Cl- transport by gastric surface epithelium in Necturus antral mucosa"],"prefix":"10.1152","volume":"264","author":[{"given":"D. I.","family":"Soybel","sequence":"first","affiliation":[{"name":"Department of Surgery, Yale University School of Medicine, West Haven,Connecticut."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. B.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Surgery, Yale University School of Medicine, West Haven,Connecticut."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Y.","family":"Cheung","sequence":"additional","affiliation":[{"name":"Department of Surgery, Yale University School of Medicine, West Haven,Connecticut."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1993.264.5.G910","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T22:49:46Z","timestamp":1567982986000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1993.264.5.G910"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1993,5,1]]}},"alternative-id":["10.1152\/ajpgi.1993.264.5.G910"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1993.264.5.g910","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,5,1]]}}}