{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T08:26:26Z","timestamp":1648974386232},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1995,3,1]]},"abstract":"<jats:p> The presence of specific receptors for gastrointestinal hormones on T cells and their involvement in the immune response are still matters of debate. We reported the effects of gastrin-cholecystokinin (CCK)-related peptides on J.RT3-T3.5 Jurkat cells. A single class of high-affinity binding sites (dissociation constant approximately 0.1 nM) for gastrin and CCK-8 was evident on these cells. These peptides dose-dependently induced a transient increase in intracellular Ca2+ concentration ([Ca2+]i), which was independent of extracellular Ca2-. L-365,260 was 150- to 300-fold more potent than L-364,718 to inhibit radiolabeled ligand binding or peptide-stimulated [Ca2+]i increase, confirming the gastrin-CCK-B nature of the receptor. Gastrin caused a rise in inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] level within 5 s of stimulation. Finally, gastrin increased interleukin (IL)-2 secretion in J.RT3-T3.5 cells. We conclude that 1) J.RT3-T3.5 cells possess \"gastrin-CCK-B type\" receptors coupled to phospholipase C activation, Ins(1,4,5)P3 formation, and Ca2+ release from intracellular Ca2+ pools, and 2) these receptors could be involved in the regulation of IL-2 production. <\/jats:p>","DOI":"10.1152\/ajpgi.1995.268.3.g522","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T10:24:33Z","timestamp":1514111073000},"page":"G522-G529","source":"Crossref","is-referenced-by-count":3,"title":["Gastrin-CCK-B type receptors on human T lymphoblastoid Jurkat cells"],"prefix":"10.1152","volume":"268","author":[{"given":"J.","family":"Dornand","sequence":"first","affiliation":[{"name":"Laboratoire de Biochimie des Membranes, Institut National de la Santeet de la Recherche Medicale CJF 92-07, Faculte de Pharmacie, Montpellier,France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Roche","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie des Membranes, Institut National de la Santeet de la Recherche Medicale CJF 92-07, Faculte de Pharmacie, Montpellier,France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Michel","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie des Membranes, Institut National de la Santeet de la Recherche Medicale CJF 92-07, Faculte de Pharmacie, Montpellier,France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. P.","family":"Bali","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie des Membranes, Institut National de la Santeet de la Recherche Medicale CJF 92-07, Faculte de Pharmacie, Montpellier,France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Cabane","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie des Membranes, Institut National de la Santeet de la Recherche Medicale CJF 92-07, Faculte de Pharmacie, Montpellier,France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Favero","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie des Membranes, Institut National de la Santeet de la Recherche Medicale CJF 92-07, Faculte de Pharmacie, Montpellier,France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Magous","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie des Membranes, Institut National de la Santeet de la Recherche Medicale CJF 92-07, Faculte de Pharmacie, Montpellier,France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1995.268.3.G522","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:07:22Z","timestamp":1567987642000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1995.268.3.G522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,3,1]]}},"alternative-id":["10.1152\/ajpgi.1995.268.3.G522"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1995.268.3.g522","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3,1]]}}}