{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T13:50:59Z","timestamp":1649080259822},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1995,11,1]]},"abstract":"<jats:p> Limited knowledge exists concerning the interleukin-1 (IL-1) receptor type (IL-1RT) mediating the potent antisecretory and gastro-protective actions of IL-1. In the present study, the gastric actions of IL-1 beta and two related mutant proteins, yIL-1 beta delta 4, an analogue that preferentially binds to IL-1-RTII, and mutant yIL-1 beta N7\/Q, an analogue that has equal affinity as IL-1 beta for IL-1RTI and IL-1RTII, have been compared. Modulation of IL-1 gastric actions were also investigated using monoclonal antibody (MAb) preparations raised against IL-1RTI or IL-1RTII. In the pylorus-ligated rat, yIL-1 beta delta 4, yIL-1 beta N7\/Q, and IL-1 beta (all at 1 microgram\/kg ip) reduced gastric acid secretion (50, 79, and 78%, respectively), indicating the importance of IL-1RTII binding for antisecretory activity. This was further substantiated in experiments using the MAb preparations, which showed that IL-1 beta (1 microgram\/kg ip) antisecretory activity was reversed by MAb IL-1RTII (10-50 micrograms\/kg sc) but not by MAb IL-1RTI (50 micrograms\/kg sc). In contrast, at dosages 10-fold higher (10 micrograms\/kg ip) than that used in the study to inhibit acid secretion, IL-1 beta and yIL-1N7\/Q equally reduced (approximately 80%) indomethacin-induced gastric damage, but yIL-1 beta delta 4 was ineffective. The results using yIL-1 beta delta 4 indicated that impairment of IL-1RTI binding capacity appeared to be paralleled by a decreased gastroprotective effect.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/ajpgi.1995.269.5.g763","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T05:56:09Z","timestamp":1514094969000},"page":"G763-G769","source":"Crossref","is-referenced-by-count":2,"title":["Gastric antisecretory and anti-ulcer actions of IL-1 in rat involve different IL-1 receptor types"],"prefix":"10.1152","volume":"269","author":[{"given":"K. G.","family":"Mugridge","sequence":"first","affiliation":[{"name":"Istituto Ricerche Immunobiologiche Siena, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Perretti","sequence":"additional","affiliation":[{"name":"Istituto Ricerche Immunobiologiche Siena, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Ghiara","sequence":"additional","affiliation":[{"name":"Istituto Ricerche Immunobiologiche Siena, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. L.","family":"Galeotti","sequence":"additional","affiliation":[{"name":"Istituto Ricerche Immunobiologiche Siena, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Melli","sequence":"additional","affiliation":[{"name":"Istituto Ricerche Immunobiologiche Siena, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Parente","sequence":"additional","affiliation":[{"name":"Istituto Ricerche Immunobiologiche Siena, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1995.269.5.G763","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T20:10:01Z","timestamp":1567973401000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1995.269.5.G763"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1995,11,1]]}},"alternative-id":["10.1152\/ajpgi.1995.269.5.G763"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1995.269.5.g763","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,11,1]]}}}