{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:46:58Z","timestamp":1791193618443,"version":"4.1.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1996,3,1]]},"abstract":"<jats:p> The present study examines the feedback control governing human cholesterol 7alpha-hydroxylase mRNA expression in the human hepatoblastoma cell line, Hep G2. Glycochenodeoxycholate (GCDC) and glycodeoxycholate, hydrophobic bile salts, decreased cholesterol 7alpha-hydroxylase mRNA levels and bile acid synthesis in a concentration-dependent (76 +\/- 8%, P&lt;0.001, and 48 +\/- 3%, P&lt;0.01, respectively) and time-dependent manner. Cholesterol 7alpha-hydroxylase mRNA levels were repressed with a half-maximal inhibitory concentration of &lt;12.5 microM by GCDC and a half-life of 30 min by 100 microM of the bile acid. The addition of actinomycin D (10 microgram\/ml) alone or in combination with GCDC (100 microM) led to similar concentration-and time-dependent suppression of cholesterol 7alpha-hydroxylase mRNA. Glycocholate (100 microM), not internalized based on lack of uptake of a fluorescent cholate analogue, had no effect on cholesterol 7alpha-hydroxylase mRNA or total bile acid synthesis. In cultures transfected with a rat cholesterol 7alpha-hydroxylase promoter construct, reporter gene activity was decreased (31%, P&lt;0.01) by GCDC (100 microM). Hep G2 cells maintain the intracellular machinery to express and rapidly regulate human cholesterol 7alpha-hydroxylase by hydrophobic bile acids. These data suggest that Hep G2 cells will support functional studies of the human cholesterol 7alpha-hydroxylase gene. <\/jats:p>","DOI":"10.1152\/ajpgi.1996.270.3.g401","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T10:54:07Z","timestamp":1514112847000},"page":"G401-G410","source":"Crossref","is-referenced-by-count":5,"title":["Hep G2 cells: a model for studies on regulation of human cholesterol 7alpha-hydroxylase at the molecular level"],"prefix":"10.1152","volume":"270","author":[{"given":"W. M.","family":"Pandak","sequence":"first","affiliation":[{"name":"Department of Medicine, Medical College of Virginia, VirginiaCommonwealth University, Richmond 23298-0711."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. T.","family":"Stravitz","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical College of Virginia, VirginiaCommonwealth University, Richmond 23298-0711."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Lucas","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical College of Virginia, VirginiaCommonwealth University, Richmond 23298-0711."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. M.","family":"Heuman","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical College of Virginia, VirginiaCommonwealth University, Richmond 23298-0711."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Y.","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Medicine, Medical College of Virginia, VirginiaCommonwealth University, Richmond 23298-0711."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1996.270.3.G401","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:12:29Z","timestamp":1567987949000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1996.270.3.G401"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,3,1]]}},"alternative-id":["10.1152\/ajpgi.1996.270.3.G401"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1996.270.3.g401","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,3,1]]}}}