{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T05:34:11Z","timestamp":1648532051454},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1996,7,1]]},"abstract":"<jats:p> We previously observed that endothelin-1 (ET-1)-induced gastric vasoconstriction is enhanced after ischemia-reperfusion. The purpose of our present study was to examine the role of nitric oxide in regulating ET-1-induced vasoconstriction under normal conditions and after ischemia-reperfusion. Using a mechanically perfused stomach segment from chloralose-anesthetized dogs, we examined 1) responses to NG-nitro-L-arginine methyl ester (L-NAME) alone and in combination with L-arginine, 2) whether L-NAME affects ET-1-induced vasoconstriction under normal conditions and after ischemia-reperfusion, and 3) if spermine NONOate inverted question mark1,3-propanediamine-N-[4-1-(3-aminopropyl)-2-hydroxy-2-nitrosohydrazi no] butyl; a nitric oxide donor inverted question mark attenuates the augmented response to ET-1 after ischemia-reperfusion. Our results show that 1) L-NAME significantly increased baseline vascular resistance and this response was reduced by L-arginine, 2) ET-1-induced vasoconstriction was enhanced by L-NAME, and 3) administration of spermine NONOate during reperfusion largely attenuated the vasoconstrictor response to ET-1 after ischemia-reperfusion. Our findings are consistent with the hypothesis that nitric oxide modulates responses to ET-1 under normal conditions, and loss of this vasodilator after ischemia-reperfusion results in an augmented response to ET-1. <\/jats:p>","DOI":"10.1152\/ajpgi.1996.271.1.g27","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T11:36:48Z","timestamp":1514115408000},"page":"G27-G35","source":"Crossref","is-referenced-by-count":0,"title":["Nitric oxide attenuates endothelin-1-induced vasoconstriction in canine stomach"],"prefix":"10.1152","volume":"271","author":[{"given":"J. G.","family":"Wood","sequence":"first","affiliation":[{"name":"Department of Surgery, University of Kansas Medical Center, KansasCity 66160, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Kansas Medical Center, KansasCity 66160, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z. Y.","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Kansas Medical Center, KansasCity 66160, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Y.","family":"Cheung","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Kansas Medical Center, KansasCity 66160, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1996.271.1.G27","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:20:17Z","timestamp":1567988417000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1996.271.1.G27"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,7,1]]}},"alternative-id":["10.1152\/ajpgi.1996.271.1.G27"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1996.271.1.g27","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,7,1]]}}}