{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T12:23:12Z","timestamp":1743423792113},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1996,8,1]]},"abstract":"<jats:p> We investigated the involvement of nitric oxide in transmural jejunal alterations induced by Trichinella spiralis (T. spiralis) infection in rats. Rats were gavaged with either saline or T.spiralis larvae, and, 1 h later, rats were treated orally with water, NG-nitro-L-arginine methyl ester (L-NAME; 30 mg\/kg), or NG-nitro-D-arginine methyl ester (D-NAME; 30 mg\/kg) on a daily basis. Although not observed in jejunum from uninfected rats, inducible nitric oxide synthase (iNOS) mRNA was present in the mucosa and neuromuscular layers of jejunum from T. spiralis-infected rats. On day 6, T. spiralis-infected rats had a 6-fold decrease in transmural nitric oxide synthase activity, an 11-fold increase in plasma nitrite, and a 7-fold elevation in transmural myeloperoxidase (MPO) activity compared with uninfected controls. Intestinal smooth muscle cell hyperplasia and hypertrophy were only detected in the infected rats. L-NAME, but not D-NAME, treatment of infected rats for 6 days caused a pronounced increase in transmural iNOS mRNA expression, coinciding with significantly increased mucosal nitric oxide synthase activity. T. spiralis numbers in L-NAME-treated rats were significantly lower compared with the other two infected groups although L-NAME had no direct effect on T. spiralis viability in vitro. Furthermore, L-NAME treatment significantly reduced plasma nitrite and jejunal MPO but not intestinal smooth muscle cell hyperplasia or hypertrophy. In contrast, D-NAME treatment of infected rats significantly enhanced intestinal smooth muscle hyperplasia and hypertrophy. Taken together, these results suggest that alterations in the T. spiralis-infected jejunum are mediated, in part, by a suppression of nitric oxide synthase activity in the inflamed jejunum. <\/jats:p>","DOI":"10.1152\/ajpgi.1996.271.2.g338","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T11:39:56Z","timestamp":1514115596000},"page":"G338-G346","source":"Crossref","is-referenced-by-count":8,"title":["Effects of oral L-NAME during Trichinella spiralis infection in rats"],"prefix":"10.1152","volume":"271","author":[{"given":"C. M.","family":"Hogaboam","sequence":"first","affiliation":[{"name":"Intestinal Disease Research Programme, McMaster University, Hamilton,Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. M.","family":"Collins","sequence":"additional","affiliation":[{"name":"Intestinal Disease Research Programme, McMaster University, Hamilton,Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. G.","family":"Blennerhassett","sequence":"additional","affiliation":[{"name":"Intestinal Disease Research Programme, McMaster University, Hamilton,Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1996.271.2.G338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:21:50Z","timestamp":1567988510000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1996.271.2.G338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,8,1]]}},"alternative-id":["10.1152\/ajpgi.1996.271.2.G338"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1996.271.2.g338","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,8,1]]}}}