{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:49:53Z","timestamp":1718653793614},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1997,1,1]]},"abstract":"<jats:p> pH regulation in isolated guinea pig pancreatic interlobular duct segments loaded with the pH-sensitive fluorophore, 5-(6)-carboxy-SNARF-1-acetoxymethyl ester (SNARF-1), was characterized by laser-scanning confocal microscopy. In HCO3(-)-free medium, intracellular pH (pHi) of duct epithelial cells fell by 0.32 +\/- 0.06 pH units in the presence of 0.5 mM amiloride and by 0.36 +\/- 0.08 pH units in the absence of Na+. In the presence of extracellular HCO3-, pHi acidified in Na(-)-free medium but not in amiloride-containing medium. Superfusion with Cl(-)-free buffers or with buffers containing 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid produced a cytosolic alkalinization of 0.13-0.22 pH units. These observations demonstrate the presence of Na+\/H+ exchange, Na(+)-HCO3- cotransport, and Cl-\/HCO3- exchange in guinea pig pancreatic ducts. pHi recovered significantly from an NH4Cl pulse in HCO3(-)-free buffers containing amiloride and carbachol (50.4%) or amiloride and secretin (40.6%). This recovery was blocked by the H(+)-adenosinetriphosphatase (H(+)-ATPase) inhibitor bafilomycin A1 and by preincubation of ducts with nocodazole or cytochalasin D. These observations suggest that a vesicular H(+)-ATPase augments Na(+)-dependent H+ extrusion during agonist-stimulated bicarbonate secretion and that activation of this transport mechanism involves cytoskeletal elements. <\/jats:p>","DOI":"10.1152\/ajpgi.1997.272.1.g124","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T06:32:03Z","timestamp":1514097123000},"page":"G124-G134","source":"Crossref","is-referenced-by-count":6,"title":["Confocal microscopic analysis of intracellular pH regulation in isolated guinea pig pancreatic ducts"],"prefix":"10.1152","volume":"272","author":[{"given":"J.","family":"de Ondarza","sequence":"first","affiliation":[{"name":"Department of Physiology, Michigan State University, East Lansing48824-1101, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. R.","family":"Hootman","sequence":"additional","affiliation":[{"name":"Department of Physiology, Michigan State University, East Lansing48824-1101, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1997.272.1.G124","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T20:20:39Z","timestamp":1567974039000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1997.272.1.G124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,1,1]]}},"alternative-id":["10.1152\/ajpgi.1997.272.1.G124"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1997.272.1.g124","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1,1]]}}}