{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:10:45Z","timestamp":1790485845140,"version":"4.1.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1997,1,1]]},"abstract":"<jats:p> Cl- secretion by pancreatic duct epithelial cells (PDEC) regulates cellular HCO3- secretion, an important component of the exocrine pancreas. In cystic fibrosis, for example, impaired function of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel results in decreased pancreatic secretion and secondary pancreatic insufficiency. Studies of ion transport by PDEC have been hindered by the lack of a practical in vitro model. We have successfully cultured nontransformed dog PDEC on Vitrogen-coated permeable membranes overlying a feeder layer of myofibroblasts and report the characterization of Cl- channels in these cells. Cl- conductance, assessed through efflux of 125I from PDEC, was stimulated by agents acting via adenosine 3',5'-cyclic monophosphate (cAMP) or cytosolic Ca2+. The Cl- conductances activated by cAMP and Ca2+ were distinct, since they were differentially inhibited by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid and, to a lesser extent, by 5-nitro-2-(3-phenylpropylamino)benzoic acid and diphenylamine-2 carboxylate. Patch-clamp studies confirmed the presence of Cl- channels activated by cAMP and Ca2+, with differential inhibition by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. The presence of CFTR Cl- channels in PDEC was confirmed by immunoblotting. These cultured PDEC are an optimal model for studies of pancreatic duct secretion. <\/jats:p>","DOI":"10.1152\/ajpgi.1997.272.1.g172","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T11:32:03Z","timestamp":1514115123000},"page":"G172-G180","source":"Crossref","is-referenced-by-count":10,"title":["Characterization of two distinct chloride channels in cultured dog pancreatic duct epithelial cells"],"prefix":"10.1152","volume":"272","author":[{"given":"T. D.","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington98108, USA. tlnguyen@u.washington.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. S.","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington98108, USA. tlnguyen@u.washington.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. W.","family":"Moody","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington98108, USA. tlnguyen@u.washington.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. R.","family":"Fox","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington98108, USA. tlnguyen@u.washington.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. E.","family":"Savard","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington98108, USA. tlnguyen@u.washington.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Kuver","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington98108, USA. tlnguyen@u.washington.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Hille","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington98108, USA. tlnguyen@u.washington.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. P.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington98108, USA. tlnguyen@u.washington.edu"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1997.272.1.G172","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:20:42Z","timestamp":1567988442000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1997.272.1.G172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,1,1]]}},"alternative-id":["10.1152\/ajpgi.1997.272.1.G172"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1997.272.1.g172","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1,1]]}}}