{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T02:29:13Z","timestamp":1648520953003},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1997,1,1]]},"abstract":"<jats:p> The distal colon absorbs K+ (JK) and secretes H+ (JH) by what is thought to be an H(+)-K(+)-adenosinetriphosphatase (H(+)-K(+)-ATPase). However, the colonic ATPase differs structurally and functionally from the gastric H(+)-K(+)-ATPase. To evaluate the link between JH and JK, JH and JK were simultaneously measured with ion-specific electrodes in segments of rat distal colon. JH and JK averaged 0.40 +\/- 0.03 and 0.30 +\/- 0.03 mu eq.h-1.cm-2 (n = 191), but JH and JK did not correlate (r = 0.005, not significant). The gastric H(+)-K+ pump inhibitors SCH-28080 (100 microM) and omeprazole (100 microM), as well as a vacuolar H(+)-ATPase inhibitor, bafilomycin A1 (10 microM), did not affect JH or JK. However, the Na(+)-K(+)-ATPase inhibitors ouabain (1 mM) and N-ethylmaleimide (10 microM) inhibited JK but not JH. Although 1 mM orthovanadate inhibited both JH and JK, at lower concentrations orthovanadate only affected JK. Furthermore, removing K+ from the medium did not affect JH. Secondary hyperaldosteronism increased both JH and JK; however, ouabain (1 mM) reduced JK but not JH. Cl(-)-free medium inhibited voltage-insensitive JH and voltage-sensitive JK. Medium pH affected JH, but that effect was contrary to the effect that pH had on Rb+ flux. These data failed to identify a relationship between JH and JK and appear to suggest that JH and JK occur by separate pathways. <\/jats:p>","DOI":"10.1152\/ajpgi.1997.272.1.g54","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T06:32:03Z","timestamp":1514097123000},"page":"G54-G62","source":"Crossref","is-referenced-by-count":0,"title":["Net H+ and K+ fluxes across the apical surface of rat distal colon"],"prefix":"10.1152","volume":"272","author":[{"given":"G. M.","family":"Feldman","sequence":"first","affiliation":[{"name":"Department of Medicine, McGuire Veterans Affairs Medical Center,Medical College of Virginia, Virginia Commonwealth University, Richmond23249, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"J. W.","family":"Ickes","sequence":"additional","affiliation":[{"name":"Department of Medicine, McGuire Veterans Affairs Medical Center,Medical College of Virginia, Virginia Commonwealth University, Richmond23249, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1997.272.1.G54","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T20:20:34Z","timestamp":1567974034000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1997.272.1.G54"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,1,1]]}},"alternative-id":["10.1152\/ajpgi.1997.272.1.G54"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1997.272.1.g54","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1,1]]}}}