{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:22:30Z","timestamp":1789694550805,"version":"4.0.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1997,3,1]]},"abstract":"<jats:p> We investigated the effects of pituitary adenylate cyclase-activating polypeptides (PACAPs) on gastroduodenal HCO(3)- secretion in anesthetized rats and characterized their effects by comparison with the effects of vasoactive intestinal polypeptide (VIP). Under urethan anesthesia, a rat proximal duodenal loop or a rat stomach mounted in an ex vivo chamber (in the absence of acid secretion) was perfused with saline, and HCO(3)- secretion was measured at pH 7.0 using a pH-stat method and by addition of 10 mM HCl. Intravenous injection of PACAP-27 stimulated HCO(3)- secretion in a dose-dependent manner in the duodenum, but not in the stomach, although this peptide had no effect on duodenal HCO(3)- secretion after intracisternal administration. The duodenal HCO(3)- stimulatory action was similarly observed after intravenous administration of PACAP-38 and VIP, and the potency of action was in the following order: PACAP-27 &gt; PACAP-38 = VIP. The duodenal HCO(3)- stimulatory action of PACAP-27 was potentiated by pretreatment with 3-isobutyl-1-methylxanthine, similar to that of prostaglandin E2, and was significantly attenuated by PACAP-(6--27) (PACAP antagonist) or Ac-Tyr1,D-Phe2-VIP (VIP antagonist) but was not affected by bilateral vagotomy or prior administration of atropine, verapamil, and indomethacin. Forskolin, the stimulator of adenylate cyclase, also increased HCO(3)- secretion in the duodenum, but not in the stomach. These results suggest that 1) PACAP is a potent stimulator of HCO(3)- secretion in the duodenum, but not in the stomach, and may be involved in the peripheral regulation of duodenal HCO(3)- secretion, 2) this action is mediated by adenosine 3',5'-cyclic monophosphate, probably through PACAP and VIP receptors, and 3) adenosine 3',5'-cyclic monophosphate is a mediator in duodenal, but not in gastric, HCO(3)- secretion. <\/jats:p>","DOI":"10.1152\/ajpgi.1997.272.3.g646","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T06:35:07Z","timestamp":1514097307000},"page":"G646-G653","source":"Crossref","is-referenced-by-count":5,"title":["PACAPs stimulate duodenal bicarbonate secretion at PACAP receptors in the rat"],"prefix":"10.1152","volume":"272","author":[{"given":"K.","family":"Takeuchi","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, KyotoPharmaceutical University, Misasagi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Takehara","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, KyotoPharmaceutical University, Misasagi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, KyotoPharmaceutical University, Misasagi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Yagi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Therapeutics, KyotoPharmaceutical University, Misasagi, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1997.272.3.G646","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T20:18:31Z","timestamp":1567973911000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1997.272.3.G646"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,3,1]]}},"alternative-id":["10.1152\/ajpgi.1997.272.3.G646"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1997.272.3.g646","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,3,1]]}}}