{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T09:51:43Z","timestamp":1724061103433},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1997,6,1]]},"abstract":"<jats:p> Hepatocytes do not express Bcl-2, a repressor of apoptosis. In contrast, cholangiocytes, which are in direct contact with bile, do express Bcl-2. Because cholestasis results in the retention of bile within hepatocytes, we reasoned cholestasis may induce hepatocellular expression of Bcl-2. Thus our aim was to determine whether hepatocytes express Bcl-2 or alter expression of other Bcl-2 family members in cholestasis using the bile duct-ligated (BDL) rat as a model of cholestasis. De novo Bcl-2 expression was observed in hepatocytes of BDL rats assessed by reverse transcriptase-polymerase chain reaction and immunoblot analysis. Immunohistochemistry demonstrated that Bcl-2 expression in hepatocytes was greater in periportal hepatocytes than pericentral hepatocytes. Expression of Bcl-x (an antiapoptotic Bcl-2 family protein) was not altered by bile duct ligation, whereas expression of Bax (a proapoptotic Bcl-2 family protein) increased slightly as determined by Northern and Western blot analyses. Bcl-2-positive hepatocytes isolated from BDL rats were resistant to induction of apoptosis by 50 microM glycochenodeoxycholate. Our results demonstrate, for the first time, expression of Bcl-2 by hepatocytes during cholestasis. We suggest that hepatocellular expression of Bcl-2 during cholestasis is an adaptive phenomenon to resist apoptosis by toxic bile salts. <\/jats:p>","DOI":"10.1152\/ajpgi.1997.272.6.g1587","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T12:19:46Z","timestamp":1514117986000},"page":"G1587-G1593","source":"Crossref","is-referenced-by-count":11,"title":["Hepatocytes in the bile duct-ligated rat express Bcl-2"],"prefix":"10.1152","volume":"272","author":[{"given":"H.","family":"Kurosawa","sequence":"first","affiliation":[{"name":"Center for Basic Research in Digestive Diseases, Mayo Clinic andFoundation, Rochester, Minnesota 55905, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. G.","family":"Que","sequence":"additional","affiliation":[{"name":"Center for Basic Research in Digestive Diseases, Mayo Clinic andFoundation, Rochester, Minnesota 55905, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. R.","family":"Roberts","sequence":"additional","affiliation":[{"name":"Center for Basic Research in Digestive Diseases, Mayo Clinic andFoundation, Rochester, Minnesota 55905, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. J.","family":"Fesmier","sequence":"additional","affiliation":[{"name":"Center for Basic Research in Digestive Diseases, Mayo Clinic andFoundation, Rochester, Minnesota 55905, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. J.","family":"Gores","sequence":"additional","affiliation":[{"name":"Center for Basic Research in Digestive Diseases, Mayo Clinic andFoundation, Rochester, Minnesota 55905, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1997.272.6.G1587","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:32:59Z","timestamp":1567989179000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1997.272.6.G1587"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1997,6,1]]}},"alternative-id":["10.1152\/ajpgi.1997.272.6.G1587"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1997.272.6.g1587","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,6,1]]}}}