{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T10:42:27Z","timestamp":1648809747726},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1997,8,1]]},"abstract":"<jats:p> In mRNA samples isolated from a gastrin (G) cell-enriched human antral cell preparation, reverse transcription-polymerase chain reaction identified products encoding part of the alpha 1-subunit of class C and D L-type voltage-dependent Ca2+ channels (VDCCs). Analysis of the polymerase chain reaction products demonstrated a 100% homology with the known human gene sequences. An antibody to the class D alpha 1-subunit immunostained 30-40% of the cultured cells; of these 90% were gastrin immunoreactive. Gastrin release stimulated by terbutaline (beta 2-agonist) and forskolin was abolished by blockade of L-type VDCCs; the effect of 3.6 mM extracellular Ca2+ was only partially reversed. In G cells the rise in intracellular Ca2+ observed in response to increasing extracellular Ca2+ from 0.5 to 3.6 mM was reduced by nitrendipine. These results indicated that human antral cells expressed class C and D L-type VDCCs. Activation of G cells with beta-adrenergic agonists required an influx of extracellular Ca2+ through these channels to stimulate gastrin release. However, activation of L-type channels was not the only mechanism underlying Ca(2+)-stimulated gastrin release. <\/jats:p>","DOI":"10.1152\/ajpgi.1997.273.2.g281","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T12:10:07Z","timestamp":1514117407000},"page":"G281-G288","source":"Crossref","is-referenced-by-count":2,"title":["L-type calcium channels regulate gastrin release from human antral G cells"],"prefix":"10.1152","volume":"273","author":[{"given":"J. M.","family":"Ray","sequence":"first","affiliation":[{"name":"Department of Physiology, University of British Columbia, Vancouver,Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. E.","family":"Squires","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of British Columbia, Vancouver,Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M.","family":"Meloche","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of British Columbia, Vancouver,Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. W.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of British Columbia, Vancouver,Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. P.","family":"Snutch","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of British Columbia, Vancouver,Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Buchan","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of British Columbia, Vancouver,Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1997.273.2.G281","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,9]],"date-time":"2019-09-09T00:27:32Z","timestamp":1567988852000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1997.273.2.G281"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,8,1]]}},"alternative-id":["10.1152\/ajpgi.1997.273.2.G281"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1997.273.2.g281","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,8,1]]}}}