{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T01:11:54Z","timestamp":1794445914399,"version":"4.9.1"},"reference-count":31,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1997,10,1]]},"abstract":"<jats:p>The effect of hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) on intestinal epithelial barrier function was examined in Caco-2 and T84 cell monolayers. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>reduced transepithelial electrical resistance (TER) of Caco-2 and T84 cell monolayers. This decrease in TER was associated with a decrease in dilution potential and an increase in [<jats:sup>3<\/jats:sup>H]mannitol permeability, suggesting an H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-induced disruption of the paracellular junctional complexes. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>administration also induced tyrosine phosphorylation of several proteins (at the molecular mass ranges of 50\u201390, 100\u2013130, and 150\u2013180 kDa) in Caco-2 cell monolayers. Phenylarsine oxide and sodium orthovanadate, inhibitors of protein tyrosine phosphatase, decreased TER and increased mannitol permeability and protein tyrosine phosphorylation (PTP). A low concentration of sodium orthovanadate also potentiated the effect of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>on TER, dilution potential, mannitol permeability, and PTP. Pretreatment with genistein (30\u2013300 \u03bcM) and tyrphostin (100 \u03bcM) inhibited the effect of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>on TER, dilution potential, mannitol permeability, and PTP. These studies show that H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>increases the epithelial permeability by disrupting paracellular junctional complexes, most likely by a PTP-dependent mechanism.<\/jats:p>","DOI":"10.1152\/ajpgi.1997.273.4.g812","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T11:56:18Z","timestamp":1514116578000},"page":"G812-G823","source":"Crossref","is-referenced-by-count":57,"title":["Oxidant-induced disruption of intestinal epithelial barrier function: role of protein tyrosine phosphorylation"],"prefix":"10.1152","volume":"273","author":[{"given":"R. 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