{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:36:31Z","timestamp":1791189391329,"version":"4.1.0"},"reference-count":42,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1997,12,1]]},"abstract":"<jats:p>The dual radiolabeled monoclonal antibody technique was used to 1) define the magnitude and kinetics of P-selectin expression in murine small intestine exposed to ischemia-reperfusion (I\/R), and 2) determine the factor(s) responsible for initiating this response. Within 10 min after release of a 20-min arterial occlusion, intestinal P-selectin expression increased two- to threefold compared with control values. Peak (4-fold) expression of P-selectin was noted at 5 h after reperfusion, returning to the control value at 24 h. The early (10\u201330 min) I\/R-induced upregulation of P-selectin appears to reflect mobilization of a preformed pool of the adhesion molecule, whereas the later (5 h) rise appears to be transcription dependent. The early increase in P-selectin expression was not inhibited by pretreatment with either oxypurinol (inhibits xanthine oxidase), diphenhydramine (H<jats:sub>1<\/jats:sub>-receptor antagonist), or MK-571 (leukotriene C<jats:sub>4<\/jats:sub>\/D<jats:sub>4<\/jats:sub>antagonist), nor was it blunted in transgenic mice expressing three times the normal level of copper-zinc superoxide dismutase or in mast cell-deficient mice. However, significant inhibition was noted after treatment with either MK-886 (5-lipoxygenase inhibitor) or a nitric oxide (NO) donor (diethylenetriamine\/NO). These findings indicate that the early I\/R-induced increase in intestinal P-selectin expression is mediated by a 5-lipoxygenase-dependent NO-inhibitable mechanism.<\/jats:p>","DOI":"10.1152\/ajpgi.1997.273.6.g1326","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T09:16:37Z","timestamp":1514106997000},"page":"G1326-G1332","source":"Crossref","is-referenced-by-count":25,"title":["Modulation of P-selectin expression in the postischemic intestinal microvasculature"],"prefix":"10.1152","volume":"273","author":[{"given":"Michael J.","family":"Eppihimer","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice","family":"Russell","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald C.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Discovery Research, Pharmacia-Upjohn Laboratories, Kalamazoo, Michigan 49001; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles J.","family":"Epstein","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of California, San Francisco, California 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Laroux","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Neil","family":"Granger","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport, Louisiana 71130;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1159\/000129042"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830241215"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117856"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.92.11.3304"},{"issue":"36","key":"B6","first-page":"H622","volume":"267","author":"Davenpeck K. L.","year":"1994","journal-title":"Am. J. Physiol."},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(94)90229-1"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.79.3.560"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.22.8044"},{"key":"B10","first-page":"191","volume":"127","author":"Galli S. J.","year":"1987","journal-title":"Am. J. Pathol."},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/343757a0"},{"issue":"20","key":"B12","first-page":"G683","volume":"257","author":"Granger D. N.","year":"1989","journal-title":"Am. J. Physiol."},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.121.3.655"},{"issue":"40","key":"B14","first-page":"H614","volume":"271","author":"Johnston B.","year":"1996","journal-title":"Am. J. Physiol."},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90636-3"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(93)81195-0"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1007\/BF02537058"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.5.879"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3109\/10739689409148272"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117952"},{"issue":"36","key":"B21","first-page":"H931","volume":"267","author":"Kubes P.","year":"1994","journal-title":"Am. J. Physiol."},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.11.4651"},{"key":"B23","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1016\/S0021-9258(19)40037-9","volume":"265","author":"Kuhn H.","year":"1990","journal-title":"J. Biol. Chem."},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.74.2.336"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.74.3.376"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115233"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.2.669"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.1.223"},{"key":"B29","doi-asserted-by":"crossref","first-page":"27739","DOI":"10.1016\/S0021-9258(18)47048-2","volume":"269","author":"Ma L. L.","year":"1994","journal-title":"J. Biol. Chem."},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0193"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)80055-J"},{"key":"B32","first-page":"589","volume":"124","author":"Mazur C.","year":"1994","journal-title":"J. Lab. Clin. Med."},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114175"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199506000-00006"},{"issue":"38","key":"B35","first-page":"H1955","volume":"269","author":"Pan\u00e9s J.","year":"1995","journal-title":"Am. J. Physiol."},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.4.749"},{"issue":"26","key":"B37","first-page":"H1740","volume":"257","author":"Suzuki M.","year":"1989","journal-title":"Am. J. Physiol."},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1097\/00019501-199309000-00008"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-199609010-00003"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.57.1.45"},{"key":"B41","doi-asserted-by":"crossref","first-page":"3487","DOI":"10.1182\/blood.V86.9.3487.bloodjournal8693487","volume":"86","author":"Yamada S.","year":"1994","journal-title":"Blood"},{"issue":"35","key":"B42","first-page":"H847","volume":"266","author":"Zimmerman B. J.","year":"1994","journal-title":"Am. J. Physiol."}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.1997.273.6.G1326","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T04:36:49Z","timestamp":1660192609000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.1997.273.6.G1326"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,12,1]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1997,12,1]]}},"alternative-id":["10.1152\/ajpgi.1997.273.6.G1326"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.1997.273.6.g1326","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,12,1]]}}}