{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T08:53:46Z","timestamp":1697187226233},"reference-count":26,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1998,6,1]]},"abstract":"<jats:p>Patients chronically infected with Helicobacter pylori are known to have hypergastrinemia. Previous studies have demonstrated the stimulation of gastrin from isolated G cells by monocytes and cytokines. The aim of this study was to determine if H. pylori can directly stimulate gastrin secretion. The secretion of gastrin from canine G cells in 48-h primary cultures was investigated using either live H. pylori bacteria or various bacterial extracts from three well-characterized strains. Whole bacterial sonic extracts and water-extracted surface proteins, but not PBS extracts, from strains 43579 (CagA<jats:sup>+<\/jats:sup>\/VacA<jats:sup>+<\/jats:sup>), 60190 (CagA<jats:sup>+<\/jats:sup>\/VacA<jats:sup>+<\/jats:sup>), and 60190:v1 (CagA<jats:sup>+<\/jats:sup>\/VacA<jats:sup>\u2212<\/jats:sup>) significantly stimulated gastrin release. Controls demonstrated that gastrin stimulation by the sonic extracts was not due to a direct toxic effect on G cells. We conclude that H. pylori produces a soluble factor(s), which can directly stimulate gastrin release in enriched canine G cell cultures. This stimulatory effect may play an important role in the H. pylori-associated hypergastrinemia and subsequent development of peptic ulcer disease.<\/jats:p>","DOI":"10.1152\/ajpgi.1998.274.6.g992","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T09:42:07Z","timestamp":1514108527000},"page":"G992-G996","source":"Crossref","is-referenced-by-count":6,"title":["<i>H. pylori<\/i>stimulates gastrin release from canine antral cells in primary culture"],"prefix":"10.1152","volume":"274","author":[{"given":"Frank S.","family":"Lehmann","sequence":"first","affiliation":[{"name":"CURE: Gastroenteric Biology Center, University of California, Los Angeles 90073;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neal","family":"Schiller","sequence":"additional","affiliation":[{"name":"University of California, Riverside, California 92521; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"Cover","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2605"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritchard","family":"Hatch","sequence":"additional","affiliation":[{"name":"University of California, Riverside, California 92521; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rein","family":"Seensalu","sequence":"additional","affiliation":[{"name":"CURE: Gastroenteric Biology Center, University of California, Los Angeles 90073;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimitoshi","family":"Kato","sequence":"additional","affiliation":[{"name":"CURE: Gastroenteric Biology Center, University of California, Los Angeles 90073;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John H.","family":"Walsh","sequence":"additional","affiliation":[{"name":"CURE: Gastroenteric Biology Center, University of California, Los Angeles 90073;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew H.","family":"Soll","sequence":"additional","affiliation":[{"name":"CURE: Gastroenteric Biology Center, University of California, Los Angeles 90073;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2362.1996.2040517.x"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.33.5.601"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)90126-J"},{"key":"B4","doi-asserted-by":"crossref","first-page":"5008","DOI":"10.1128\/IAI.61.12.5008-5012.1993","volume":"61","author":"Cover T. 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