{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:24:31Z","timestamp":1758925471633,"version":"3.41.0"},"reference-count":31,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1998,7,1]]},"abstract":"<jats:p>Extracellular triphosphate nucleotides, such as ATP, may regulate various cellular functions through specific cell surface receptors. We examine in this report the different secretory effects of ATP and analogs on nontransformed dog pancreatic duct epithelial cells (PDEC). We observed that 1) ATP, UTP, adenosine 5\u2032- O-(3-thiotriphosphate), and, to a lesser extent, \u03b2,\u03b3-methylene-ATP, but not adenosine, stimulated<jats:sup>125<\/jats:sup>I<jats:sup>\u2212<\/jats:sup>efflux from PDEC, suggesting a primary role for P<jats:sub>2Y2<\/jats:sub>receptors, 2) ATP-stimulated<jats:sup>125<\/jats:sup>I<jats:sup>\u2212<\/jats:sup>efflux was inhibited by 5-nitro-2-(3-phenylpropylamino)benzoic acid, diphenylamine-2-carboxylate, and DIDS, suggesting mediation through Ca<jats:sup>2+<\/jats:sup>-activated Cl<jats:sup>\u2212<\/jats:sup>channels, 3) ATP stimulated an<jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup>efflux sensitive to BaCl<jats:sub>2<\/jats:sub>and charybdotoxin, thus likely occurring through Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup>channels, 4) serosal or luminal addition of UTP activated apical Cl<jats:sup>\u2212<\/jats:sup>conductance and basolateral K<jats:sup>+<\/jats:sup>conductance when nystatin-permeabilized PDEC were studied in an Ussing chamber, suggesting the expression of P<jats:sub>2Y2<\/jats:sub>receptors on both sides of the cell, 5) ATP stimulated mucin secretion, and 6) ATP increases intracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>). In conclusion, ATP and UTP interact with P<jats:sub>2Y2<\/jats:sub>receptors on nontransformed PDEC to increase [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>, stimulate mucin secretion, and activate ion conductances; these findings have implications for pancreatic exocrine function in both health and disease, such as cystic fibrosis.<\/jats:p>","DOI":"10.1152\/ajpgi.1998.275.1.g104","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T09:43:26Z","timestamp":1514108606000},"page":"G104-G113","source":"Crossref","is-referenced-by-count":18,"title":["Secretory effects of ATP on nontransformed dog pancreatic duct epithelial cells"],"prefix":"10.1152","volume":"275","author":[{"given":"Toan D.","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Washington, and Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark W.","family":"Moody","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, and Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher E.","family":"Savard","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, and Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sum P.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, and Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3160943"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5304.1324"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.1.312"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.153.6.8665037"},{"issue":"40","key":"B5","first-page":"C468","volume":"271","author":"Chan H. 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