{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:05:32Z","timestamp":1758924332093,"version":"3.41.0"},"reference-count":23,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1998,7,1]]},"abstract":"<jats:p>Histamine affects pancreatic secretion, but its direct action on ion transport by pancreatic duct epithelial cells (PDEC) has not been defined. We now characterize the secretory effects of histamine on cultured, well-differentiated, and nontransformed dog PDEC. Histamine stimulated, in a concentration-dependent manner (1\u2013100 \u03bcM), a cellular<jats:sup>125<\/jats:sup>I<jats:sup>\u2212<\/jats:sup>efflux that was inhibited by 500 \u03bcM 5-nitro-2-(3-phenylpropylamino)benzoic acid, 2.5 mM diphenylamine-2-carboxylate, and 500 \u03bcM DIDS and thus mediated through Ca<jats:sup>2+<\/jats:sup>-activated Cl<jats:sup>\u2212<\/jats:sup>channels. Histamine-stimulated<jats:sup>125<\/jats:sup>I<jats:sup>\u2212<\/jats:sup>efflux was 1) inhibited by 100 \u03bcM diphenhydramine, an H<jats:sub>1<\/jats:sub>receptor antagonist, 2) resistant to 1 mM cimetidine, an H<jats:sub>2<\/jats:sub>receptor antagonist, 3) not reproduced by 1 mM dimaprit, an H<jats:sub>2<\/jats:sub>agonist, and 4) inhibited by 50 \u03bcM 1,2-bis(2-aminophenoxy)ethane- N, N, N\u2032, N\u2032-tetraacetic acid-AM, a Ca<jats:sup>2+<\/jats:sup>chelator, suggesting that it was mediated through H<jats:sub>1<\/jats:sub>receptors acting via increased cytosolic Ca<jats:sup>2+<\/jats:sup>. Histamine also stimulated a<jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup>efflux that was sensitive to 100 nM charybdotoxin and thus mediated through Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup>channels. When PDEC monolayers were studied in Ussing chambers, a short-circuit current of 21.7 \u00b1 3.1 \u03bcA\/cm<jats:sup>2<\/jats:sup>was stimulated by 100 \u03bcM histamine. This effect was inhibited by diphenhydramine but not cimetidine, was not reproduced with dimaprit, and was observed only after serosal addition of histamine, suggesting that it was mediated by basolateral H<jats:sub>1<\/jats:sub>receptors on PDEC. In conclusion, histamine, acting through basolateral H<jats:sub>1<\/jats:sub>receptors, activates both Ca<jats:sup>2+<\/jats:sup>-activated Cl<jats:sup>\u2212<\/jats:sup>and K<jats:sup>+<\/jats:sup>channels; in this manner, it may regulate PDEC secretion in normal or inflamed pancreas.<\/jats:p>","DOI":"10.1152\/ajpgi.1998.275.1.g76","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T09:43:26Z","timestamp":1514108606000},"page":"G76-G84","source":"Crossref","is-referenced-by-count":7,"title":["Histamine stimulates ion transport by dog pancreatic duct epithelial cells through H<sub>1<\/sub>receptors"],"prefix":"10.1152","volume":"275","author":[{"given":"Toan D.","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Washington, and Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles N.","family":"Okolo","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, and Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark W.","family":"Moody","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, and Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01983142"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/104.12.1610"},{"key":"B3","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0031-6997(25)00039-0","volume":"42","author":"Hill S. 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