{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T23:12:30Z","timestamp":1718061150982},"reference-count":52,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1998,8,1]]},"abstract":"<jats:p>The role of calcitriol in stimulating intestinal active Ca<jats:sup>2+<\/jats:sup>absorption during postnatal life was studied in newborn, suckling, and weaned control (Con) piglets and piglets suffering from inherited calcitriol deficiency (Def piglets). In addition, a group of Def piglets was treated with vitamin D<jats:sub>3<\/jats:sub>(Def-D<jats:sub>3<\/jats:sub>piglets), which normalized plasma calcitriol levels. Regardless of age, duodenal calbindin-D<jats:sub>9k<\/jats:sub>concentrations ranged between 1,839 and 2,846 \u03bcg\/g mucosa in Con piglets, between 821 and 1,219 \u03bcg\/g mucosa in Def piglets, and between 2,960 and 3,692 \u03bcg\/g mucosa in Def-D<jats:sub>3<\/jats:sub>animals. In weaned animals, active Ca<jats:sup>2+<\/jats:sup>absorption as calculated from in vitro<jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup>flux rate measurements in Ussing chambers could be related to calbindin-D<jats:sub>9k<\/jats:sub>levels. Thus active Ca<jats:sup>2+<\/jats:sup>absorption was completely absent in Def animals but was reconstituted in Def-D<jats:sub>3<\/jats:sub>animals. In contrast, in newborn Def piglets active Ca<jats:sup>2+<\/jats:sup>absorption functioned normally despite the low plasma calcitriol and mucosal calbindin-D<jats:sub>9k<\/jats:sub>levels and could not be affected by treatment with vitamin D<jats:sub>3<\/jats:sub>. Similar results were obtained from suckling Def piglets. The microtubule-disrupting agent colchicine caused significant inhibition of transepithelial net Ca<jats:sup>2+<\/jats:sup>absorption in duodenal epithelia from newborn piglets without exerting an effect in suckling and weaned animals. Colchicine had no effect on Ca<jats:sup>2+<\/jats:sup>uptake across the brush border membrane of mucosal enterocytes or on glucose-dependent electrogenic net ion flux rates in duodenal preparations from newborn Con piglets. In conclusion, our findings reveal intestinal active Ca<jats:sup>2+<\/jats:sup>absorption during early postnatal life of pigs that involves calcitriol-independent mechanisms and that may include intact microtubule actions.<\/jats:p>","DOI":"10.1152\/ajpgi.1998.275.2.g305","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T09:38:29Z","timestamp":1514108309000},"page":"G305-G313","source":"Crossref","is-referenced-by-count":3,"title":["Duodenal Ca<sup>2+<\/sup>absorption is not stimulated by calcitriol during early postnatal development of pigs"],"prefix":"10.1152","volume":"275","author":[{"given":"Bernd","family":"Schroeder","sequence":"first","affiliation":[{"name":"Department of Physiology, School of Veterinary Medicine, D-30173 Hannover; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Regina","family":"Dahl","sequence":"additional","affiliation":[{"name":"Department of Veterinary Physiology, Justus Liebig University, 35392 Giessen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerhard","family":"Breves","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Veterinary Medicine, D-30173 Hannover; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(95)00078-E"},{"key":"B2","first-page":"94","volume":"16","author":"Bronner F.","year":"1990","journal-title":"Miner. 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