{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:28:05Z","timestamp":1761060485017},"reference-count":42,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1999,2,1]]},"abstract":"<jats:p>The potency of in vitro-added corticosteroids to stimulate electrogenic Na<jats:sup>+<\/jats:sup>absorption ( J<jats:sub>Na<\/jats:sub>, the Na<jats:sup>+<\/jats:sup>absorptive short-circuit current blockable by 10<jats:sup>\u22124<\/jats:sup>M amiloride) was determined in rat late distal colon. J<jats:sub>Na<\/jats:sub>was determined 8 h after steroid addition from the drop in short-circuit current caused by 10<jats:sup>\u22124<\/jats:sup>M amiloride. The concentration dependency of J<jats:sub>Na<\/jats:sub>was obtained for seven corticosteroids and compared with that established for aldosterone. Apparent mineralocorticoid potencies as determined from apparent Michaelis-Menten constant ( K<jats:sub>m<\/jats:sub>) values were as follows: aldosterone 1.2 nM \u226b RU-28362 20 nM = deoxycorticosterone 20 nM &gt; deoxycortisol 36 nM \u2265 dexamethasone 37 nM \u226b corticosterone 170 nM &gt; cortisol 210 nM. These steroids exhibited V<jats:sub>max<\/jats:sub>values of 9\u201313 \u03bcmol \u22c5 h<jats:sup>\u22121<\/jats:sup>\u22c5 cm<jats:sup>\u22122<\/jats:sup>and similar concentration dependencies. Hill coefficients were between 1.6 and 2.1, suggesting cooperative effects between activated receptors. We conclude that corticosteroids exhibit graded mineralocorticoid potency instead of a sharp partition into exclusive groups of mineralocorticoid and nonmineralocorticoid hormones. The low apparent K<jats:sub>m<\/jats:sub>value of RU-28362 for mineralocorticoid action and the need for high concentrations of the mineralocorticoid antagonist mespirenone to block this response indicated that J<jats:sub>Na<\/jats:sub>in a native mammalian epithelium can be mediated by the glucocorticoid receptor. Glucocorticoid receptor-specific amounts of RU-28362 in combination with mineralocorticoid receptor-specific amounts of aldosterone or of the mineralocorticoid antagonist spironolactone showed cooperative action, suggesting a heterodimeric activation of J<jats:sub>Na<\/jats:sub>by the glucocorticoid receptor and mineralocorticoid receptor.<\/jats:p>","DOI":"10.1152\/ajpgi.1999.276.2.g491","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T09:51:09Z","timestamp":1514109069000},"page":"G491-G498","source":"Crossref","is-referenced-by-count":7,"title":["Electrogenic Na<sup>+<\/sup>transport in rat late distal colon by natural and synthetic glucocorticosteroids"],"prefix":"10.1152","volume":"276","author":[{"given":"Ingo","family":"Grotjohann","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Klinische Physiologie and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rg-Dieter","family":"Schulzke","sequence":"additional","affiliation":[{"name":"Medizinische Klinik I Gastroenterologie und Infektiologie, Universit\u00e4tsklinikum Benjamin Franklin, Freie Universit\u00e4t Berlin, D-12200 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Fromm","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Klinische Physiologie and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113079"},{"issue":"24","key":"B2","first-page":"F1235","volume":"255","author":"Bastl C. 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