{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T19:06:48Z","timestamp":1648753608998},"reference-count":24,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[1999,2,1]]},"abstract":"<jats:p> We examined the mechanisms of cellular Na<jats:sup>+<\/jats:sup> transport, both Cl<jats:sup>\u2212<\/jats:sup> dependent and Cl<jats:sup>\u2212<\/jats:sup> independent, in the mammalian esophageal epithelium. Rabbit esophageal epithelium was dissected from its muscular layers and mounted in a modified Ussing chamber for impalement with ion-selective microelectrodes. In bicarbonate Ringer, transepithelial potential difference was \u221214.9 \u00b1 0.9 mV, the transepithelial resistance ( R <jats:sub>TE<\/jats:sub>) was 1,879 \u00b1 142 \u03a9 \u22c5 cm<jats:sup>2<\/jats:sup>, the basolateral membrane potential difference ( V <jats:sub>mBL<\/jats:sub>) was \u221253 \u00b1 1.5 mV, and the intracellular activity of Na<jats:sup>+<\/jats:sup>([Formula: see text]) was 24.6 \u00b1 2.1 mM. Removal of Na<jats:sup>+<\/jats:sup> and Cl<jats:sup>\u2212<\/jats:sup> from the serosal and luminal baths decreased [Formula: see text] to 6.6 \u00b1 0.6 mM. Readdition of Na<jats:sup>+<\/jats:sup> to the serosal bath in the absence of Cl<jats:sup>\u2212<\/jats:sup> increased[Formula: see text] by 21.8 \u00b1 3.0 mM, whereas V <jats:sub>mBL<\/jats:sub> and R <jats:sub>TE<\/jats:sub> remained unchanged. When serosal Na<jats:sup>+<\/jats:sup> was readded in the presence of amiloride the increase in[Formula: see text] and the rate of Na<jats:sup>+<\/jats:sup> entry were decreased by \u223c50%. 5-( N-ethyl- N-isopropyl)amiloride mimicked the effect of amiloride, whereas phenamil did not. Subsequent readdition of Cl<jats:sup>\u2212<\/jats:sup> to the serosal bath further increased [Formula: see text] by 4.4 \u00b1 1.9 mM. When the cells were acid loaded by pretreatment with[Formula: see text] in nominally[Formula: see text]-free Ringer, intracellular pH measurements showed a pH<jats:sub>i<\/jats:sub> recovery that is dependent on the presence of Na<jats:sup>+<\/jats:sup> in the serosal bath and that can be blocked by amiloride. These data indicate that esophageal epithelial cells possess a Na<jats:sup>+<\/jats:sup>-dependent, amiloride-sensitive electroneutral mechanism for Na<jats:sup>+<\/jats:sup>entry consistent with the presence of a basolateral Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> exchanger. The ability of Cl<jats:sup>\u2212<\/jats:sup> to further enhance Na<jats:sup>+<\/jats:sup> entry supports the existence of at least one additional Cl<jats:sup>\u2212<\/jats:sup>-dependent component of basolateral Na<jats:sup>+<\/jats:sup>entry. <\/jats:p>","DOI":"10.1152\/ajpgi.1999.276.2.g507","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T04:51:09Z","timestamp":1514091069000},"page":"G507-G517","source":"Crossref","is-referenced-by-count":3,"title":["Mechanisms of basolateral Na<sup>+<\/sup> transport  in rabbit esophageal epithelial cells"],"prefix":"10.1152","volume":"276","author":[{"given":"Solange","family":"Abdulnour-Nakhoul","sequence":"first","affiliation":[{"name":"Departments of Medicine and Physiology, Tulane University School of Medicine and Veterans Affairs Medical Center, New Orleans, Louisiana 70112-2699"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serhat","family":"Bor","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Physiology, Tulane University School of Medicine and Veterans Affairs Medical Center, New Orleans, Louisiana 70112-2699"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nese","family":"Imeryuz","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Physiology, Tulane University School of Medicine and Veterans Affairs Medical Center, New Orleans, Louisiana 70112-2699"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roy C.","family":"Orlando","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Physiology, Tulane University School of Medicine and Veterans Affairs Medical Center, New Orleans, Louisiana 70112-2699"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)80209-6"},{"issue":"36","key":"B2","first-page":"F725","volume":"267","author":"Abdulnour-Nakhoul S.","year":"1994","journal-title":"Am. 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