{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:40:07Z","timestamp":1751175607486,"version":"3.41.0"},"reference-count":26,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2000,3,1]]},"abstract":"<jats:p>To determine the role of carbonic anhydrase (CA) in colonic electrolyte transport, we studied Car-2<jats:sup>0<\/jats:sup>mice, mutants deficient in cytosolic CA II. Ion fluxes were measured under short-circuit conditions in an Ussing chamber. CA was analyzed by assay and Western blots. In Car-2<jats:sup>0<\/jats:sup>mouse colonic mucosa, total CA activity was reduced 80% and cytosolic CA I and membrane-bound CA IV activities were not increased. Western blots confirmed the absence of CA II in Car-2<jats:sup>0<\/jats:sup>mice. Normal mouse distal colon exhibited net Na<jats:sup>+<\/jats:sup>and Cl<jats:sup>\u2212<\/jats:sup>absorption, a serosa-positive PD, and was specifically sensitive to pH. Decrease in pH stimulated active Na<jats:sup>+<\/jats:sup>and Cl<jats:sup>\u2212<\/jats:sup>absorption whether it was caused by increasing solution [Formula: see text], reducing [Formula: see text] concentration, or reducing pH in CO<jats:sub>2<\/jats:sub>\/[Formula: see text]-free HEPES-Ringer solution. Membrane-permeant methazolamide, but not impermeant benzolamide, at 0.1 mM prevented the effects of pH. Car-2<jats:sup>0<\/jats:sup>mice exhibited similar basal transport rates and responses to pH and CA inhibitors. We conclude that basal and pH-stimulated colonic electrolyte absorption in mice requires CA I. 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