{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:40:06Z","timestamp":1751175606824,"version":"3.41.0"},"reference-count":45,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2000,4,1]]},"abstract":"<jats:p>The effect of ANG II on mucosal ion transport and localization of ANG type 1 receptor (AT<jats:sub>1<\/jats:sub>R) in the guinea pig distal colon was investigated. Submucosal\/mucosal segments were mounted in Ussing flux chambers, and short-circuit current ( I<jats:sub>sc<\/jats:sub>) was measured as an index of ion transport. Serosal addition of ANG II produced a concentration-dependent (10<jats:sup>\u2212<\/jats:sup><jats:sup>9<\/jats:sup>\u201310<jats:sup>\u2212<\/jats:sup><jats:sup>5<\/jats:sup>M) increase in I<jats:sub>sc<\/jats:sub>. The maximal response was observed at 10<jats:sup>\u2212<\/jats:sup><jats:sup>6<\/jats:sup>M; the increase in I<jats:sub>sc<\/jats:sub>was 164.4 \u00b1 11.8 \u03bcA\/cm<jats:sup>2<\/jats:sup>. The ANG II (10<jats:sup>\u2212<\/jats:sup><jats:sup>6<\/jats:sup>M)-evoked response was mainly due to Cl<jats:sup>\u2212<\/jats:sup>secretion. Tetrodotoxin, atropine, the neurokinin type 1 receptor antagonist FK-888, and piroxicam significantly reduced the ANG II (10<jats:sup>\u2212<\/jats:sup><jats:sup>6<\/jats:sup>M)-evoked response to 28, 45, 58, and 16% of control, respectively. Pretreatment with prostaglandin E<jats:sub>2<\/jats:sub>(10<jats:sup>\u2212<\/jats:sup><jats:sup>5<\/jats:sup>M) resulted in a threefold increase in the ANG II-evoked response. The AT<jats:sub>1<\/jats:sub>R antagonist FR-130739 completely blocked ANG II (10<jats:sup>\u2212<\/jats:sup><jats:sup>6<\/jats:sup>M)-evoked responses, whereas the ANG type 2 receptor antagonist PD-123319 had no effect. Localization of AT<jats:sub>1<\/jats:sub>R was determined by immunohistochemistry. In the immunohistochemical study, AT<jats:sub>1<\/jats:sub>R-immunopositive cells were distributed clearly in enteric nerves and moderately in surface epithelial cells. These results suggest that ANG II-evoked electrogenic Cl<jats:sup>\u2212<\/jats:sup>secretion may involve submucosal cholinergic and tachykinergic neurons and prostanoid synthesis pathways through AT<jats:sub>1<\/jats:sub>R on the submucosal plexus and surface epithelial cells in guinea pig distal colon.<\/jats:p>","DOI":"10.1152\/ajpgi.2000.278.4.g625","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:10:01Z","timestamp":1514128201000},"page":"G625-G634","source":"Crossref","is-referenced-by-count":12,"title":["Mode of action of ANG II on ion transport in guinea pig distal colon"],"prefix":"10.1152","volume":"278","author":[{"given":"Yutaka","family":"Hosoda","sequence":"first","affiliation":[{"name":"Laboratory of Environmental Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka 422-8526; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adi","family":"Winarto","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy, Hokkaido University, Sapporo 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiko","family":"Iwanaga","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Anatomy, Hokkaido University, Sapporo 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsukazu","family":"Kuwahara","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Physiology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka 422-8526; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1993.264.2.G252"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1991.261.3.G443"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112566"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1210\/endo-121-5-1616"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119296"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1984.246.3.G263"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1984.246.4.G372"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1997.272.2.G238"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1987.tb08969.x"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-6980(97)00015-4"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.29.1.510"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1997.273.1.R28"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.31.1.473"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.263.4.C838"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1989.257.4.G504"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1988.255.4.F563"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1992.263.1.G91"},{"key":"B19","first-page":"62","volume":"12","author":"Greger R","year":"1997","journal-title":"News Physiol Sci"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.262.6.C1392"},{"key":"B21","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0022-3565(25)12248-9","volume":"276","author":"Hatch M","year":"1996","journal-title":"J Pharmacol Exp Ther"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050694"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/0922-4106(93)90122-P"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80071-N"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(90)91088-N"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1998.275.2.R515"},{"key":"B27","first-page":"3925","volume":"83","author":"Karlsson C","year":"1998","journal-title":"J. Clin Endocrinol Metab"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.5.C1294"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.1252"},{"key":"B30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0022-3565(25)13314-4","volume":"252","author":"Kuwahara A","year":"1990","journal-title":"J Pharmacol Exp Ther"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1998.275.4.G829"},{"key":"B32","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1016\/S0022-3565(25)39603-5","volume":"267","author":"Leung E","year":"1993","journal-title":"J Pharmacol Exp Ther"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1981.240.1.G17"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1985.249.1.G3"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1007\/BF00704157"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1997.273.6.G1341"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(93)90403-U"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.4.C983"},{"key":"B39","doi-asserted-by":"crossref","first-page":"9455","DOI":"10.1016\/S0021-9258(19)50109-0","volume":"267","author":"Sandberg K","year":"1992","journal-title":"J Biol Chem"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1177\/34.7.3519756"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1993.265.1.G21"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374465"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)00728-P"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1997.v113.pm9352857"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)92133-5"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1985.249.4.G457"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.2000.278.4.G625","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:13:24Z","timestamp":1751174004000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.2000.278.4.G625"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,4,1]]},"references-count":45,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2000,4,1]]}},"alternative-id":["10.1152\/ajpgi.2000.278.4.G625"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.2000.278.4.g625","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"type":"print","value":"0193-1857"},{"type":"electronic","value":"1522-1547"}],"subject":[],"published":{"date-parts":[[2000,4,1]]}}}