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Retrograde infusion with significant elevations in pressure led to paracellular leakage of lanthanum chloride, radiopaque dye, and [<jats:sup>14<\/jats:sup>C]sucrose with rapid systemic redistribution via sinusoidal and subsequent hepatic venous drainage. Chronic extrahepatic bile duct obstruction resulted in significantly smaller peak intrabiliary pressures and lower levels of paracellular leakage. These findings indicate that under both normostatic and cholestatic conditions elevated intrabiliary volumes\/pressures result in an acute pressure-dependent physical opening of tight junctions, permitting the movement of infusate from the intrabiliary space into the subepithelial tissue compartment. Control of intraluminal pressure may potentially permit the selective delivery of macromolecules &gt;18\u201320\u00c5 in diameter to specific histological compartments.<\/jats:p>","DOI":"10.1152\/ajpgi.2000.279.1.g49","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:30:49Z","timestamp":1514129449000},"page":"G49-G66","source":"Crossref","is-referenced-by-count":16,"title":["Manometric changes during retrograde biliary infusion in mice"],"prefix":"10.1152","volume":"279","author":[{"given":"Stephen M.","family":"Wiener","sequence":"first","affiliation":[{"name":"Molecular Hematology Branch and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Robert F.","family":"Hoyt","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Medicine and Surgery, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda 20892;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John 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