{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:00:20Z","timestamp":1791273620595,"version":"4.1.0"},"reference-count":54,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2000,8,1]]},"abstract":"<jats:p>Long-chain fatty acids are potent stimulants of secretin and CCK release. The cellular mechanisms of fatty acid-stimulated secretion of these two hormones are not clear. We studied the stimulatory effect and mechanism of sodium oleate (SO) on secretin- and CCK-producing cells. SO stimulated the release of secretin or CCK from isolated rat mucosal cell preparations enriched in either secretin- or CCK-producing cells, respectively. SO also time- and dose-dependently stimulated secretin and CCK release from STC-1 cells. In STC-1 cells, SO-stimulated secretin and CCK release was potentiated by IBMX and inhibited by a protein kinase A-selective inhibitor and a cAMP-specific antagonist. SO-stimulated releases of the two hormones were also inhibited by downregulation or inhibitors of protein kinase C, a calmodulin antagonist and an inhibitor of calmodulin-dependent protein kinase II. Chelating of extracellular Ca<jats:sup>2+<\/jats:sup>or addition of an L-type calcium channel blocker diminished SO-stimulated hormone releases. SO caused an increase in intracellular Ca<jats:sup>2+<\/jats:sup>concentration that was partially reversed by diltiazem but had no effect on production of cAMP, cGMP, or inositol-1,4,5-triphosphate. These results indicate that SO acts on secretin- and CCK-producing cells. Its stimulatory effect is potentiated by endogenous protein kinase A and mediated by activation of Ca<jats:sup>2+<\/jats:sup>influx through the L-type channels and of protein kinase C and Ca<jats:sup>2+<\/jats:sup>\/calmodulin-dependent protein kinase II.<\/jats:p>","DOI":"10.1152\/ajpgi.2000.279.2.g295","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:26:08Z","timestamp":1514129168000},"page":"G295-G303","source":"Crossref","is-referenced-by-count":22,"title":["Cellular mechanism of sodium oleate-stimulated secretion of cholecystokinin and secretin"],"prefix":"10.1152","volume":"279","author":[{"given":"Cecilia H.","family":"Chang","sequence":"first","affiliation":[{"name":"Konar Center for Digestive and Liver Diseases, Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William 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