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Addition of nutrient 10 mM NH<jats:sub>4<\/jats:sub>Cl (calculated NH<jats:sub>3<\/jats:sub>concentration = 92.5 \u03bcM and NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup>concentration = 9.91 mM) inhibited acid secretion within 30 min. Inhibition of acid secretion did not occur by blockade of basolateral K<jats:sup>+<\/jats:sup>channel activity or ion transport activity or by neutralization of the luminal solution. Although ATP depletion occurred in the presence of NH<jats:sub>4<\/jats:sub>Cl, the magnitude of ATP depletion in 30 min was not sufficient to inhibit stimulated acid secretion. By comparing the effect of NH<jats:sub>4<\/jats:sub>Cl on the resistance of inhibited or stimulated tissues, we demonstrate that NH<jats:sub>4<\/jats:sub>Cl acts specifically on stimulated tissues. We propose that NH<jats:sub>4<\/jats:sub>Cl blocks activity of an apical K<jats:sup>+<\/jats:sup>channel present in stimulated oxyntic cells. Our data suggest that the activity of this channel is important for the regulation of acid secretion in bullfrog oxyntic cells.<\/jats:p>","DOI":"10.1152\/ajpgi.2000.279.2.g400","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:26:08Z","timestamp":1514129168000},"page":"G400-G410","source":"Crossref","is-referenced-by-count":18,"title":["NH<sub>4<\/sub>Cl inhibition of acid secretion: possible involvement of an apical K<sup>+<\/sup>channel in bullfrog oxyntic cells"],"prefix":"10.1152","volume":"279","author":[{"given":"Susan J.","family":"Hagen","sequence":"first","affiliation":[{"name":"Department of Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts 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