{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T09:29:56Z","timestamp":1770974996412,"version":"3.50.1"},"reference-count":66,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2001,4,1]]},"abstract":"<jats:p>Recent studies have detected significant elevations of interleukin (IL)-5 mRNA in the liver parenchyma of patients with both primary biliary cirrhosis and acute rejection after liver transplantation. In both of these disorders, intrahepatic biliary epithelial cells (BECs) are the targets of injury. We hypothesized that BECs may themselves express IL-5 receptors that may modulate key biliary functions. RNAs coding for IL-5\u03b1 and -\u03b2 receptors were amplified by RT\/PCR from a biliary cell line derived from a human cholangiocarcinoma (Mz-ChA-1) and verified by DNA sequencing. IL-5 receptor distribution was detected immunocytochemically on Mz-ChA-1 cells, immortalized murine BEC, bile duct-ligated rat liver, and isolated cholangiocytes. Patch-clamp studies on Mz-ChA-1 cells showed that IL-5 inhibits 5\u2032- N-ethylcarboxamidoadenosine-stimulated chloride currents. Additional functional studies showed that IL-5 inhibits secretin-induced bile flow. We conclude that BECs express IL-5 receptors and that IL-5 modulates BEC chloride currents and fluid secretion. Since IL-5 has previously been associated with cholestatic liver disease, we speculate that IL-5 may contribute to liver injury through its effects on biliary secretion.<\/jats:p>","DOI":"10.1152\/ajpgi.2001.280.4.g738","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:41:03Z","timestamp":1514130063000},"page":"G738-G745","source":"Crossref","is-referenced-by-count":14,"title":["Interleukin-5 inhibition of biliary cell chloride currents and bile flow"],"prefix":"10.1152","volume":"280","author":[{"given":"James M.","family":"McGill","sequence":"first","affiliation":[{"name":"Roudebush Veterans Affairs Medical Center and"},{"name":"Departments of Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret S.","family":"Yen","sequence":"additional","affiliation":[{"name":"Departments of Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar W.","family":"Cummings","sequence":"additional","affiliation":[{"name":"Pathology,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianfranco","family":"Alpini","sequence":"additional","affiliation":[{"name":"Scott and White Hospital and The Texas Health Science Center College of Medicine and"},{"name":"Central Texas Veterans Health Care System, Temple, Texas 76502"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gene","family":"LeSage","sequence":"additional","affiliation":[{"name":"Scott and White Hospital and The Texas Health Science Center College of Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen E.","family":"Pollok","sequence":"additional","affiliation":[{"name":"Pediatric Hematology\/Oncology, Herman B. 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