{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:07:14Z","timestamp":1791184034399,"version":"4.1.0"},"reference-count":42,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2001,5,1]]},"abstract":"<jats:p>The oxidant source in alcohol-induced liver disease remains unclear. NADPH oxidase (mainly in liver Kupffer cells and infiltrating neutrophils) could be a potential free radical source. We aimed to determine if NADPH oxidase inhibitor diphenyleneiodonium sulfate (DPI) affects nuclear factor-\u03baB (NF-\u03baB) activation, liver tumor necrosis factor-\u03b1 (TNF-\u03b1) mRNA expression, and early alcohol-induced liver injury in rats. Male Wistar rats were fed high-fat liquid diets with or without ethanol (10\u201316 g \u00b7 kg<jats:sup>\u22121<\/jats:sup>\u00b7 day<jats:sup>\u22121<\/jats:sup>) continuously for up to 4 wk, using the Tsukamoto-French intragastric enteral feeding protocol. DPI or saline vehicle was administered by subcutaneous injection for 4 wk. Mean urine ethanol concentrations were similar between the ethanol- and ethanol plus DPI-treated groups. Enteral ethanol feeding caused severe fat accumulation, mild inflammation, and necrosis in the liver (pathology score, 4.3 \u00b1 0.3). In contrast, DPI significantly blunted these changes (pathology score, 0.8 \u00b1 0.4). Enteral ethanol administration for 4 wk also significantly increased free radical adduct formation, NF-\u03baB activity, and TNF-\u03b1 expression in the liver. DPI almost completely blunted these parameters. These results indicate that DPI prevents early alcohol-induced liver injury, most likely by inhibiting free radical formation via NADPH oxidase, thereby preventing NF-\u03baB activation and TNF-\u03b1 mRNA expression in the liver.<\/jats:p>","DOI":"10.1152\/ajpgi.2001.280.5.g1005","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:41:20Z","timestamp":1514130080000},"page":"G1005-G1012","source":"Crossref","is-referenced-by-count":120,"title":["Diphenyleneiodonium sulfate, an NADPH oxidase inhibitor, prevents early alcohol-induced liver injury in the rat"],"prefix":"10.1152","volume":"280","author":[{"given":"Hiroshi","family":"Kono","sequence":"first","affiliation":[{"name":"Laboratory of Hepatobiology and Toxicology, Department of Pharmacology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Rusyn","sequence":"additional","affiliation":[{"name":"Laboratory of Hepatobiology and Toxicology, Department of 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