{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T16:09:48Z","timestamp":1773850188104,"version":"3.50.1"},"reference-count":53,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2001,10,1]]},"abstract":"<jats:p>Short-circuit current ( I<jats:sub>sc<\/jats:sub>) and transepithelial conductance ( G<jats:sub>t<\/jats:sub>) were measured in guinea pig distal colonic mucosa isolated from submucosa and underlying muscle layers. Indomethacin (2 \u03bcM) and NS-398 (2 \u03bcM) were added to suppress endogenous production of prostanoids. Serosal addition of PGE<jats:sub>2<\/jats:sub>(10 nM) stimulated negative I<jats:sub>sc<\/jats:sub>consistent with K secretion, and concentrations &gt;30 nM stimulated positive I<jats:sub>sc<\/jats:sub>consistent with Cl secretion. PGE<jats:sub>2<\/jats:sub>also stimulated G<jats:sub>t<\/jats:sub>at low and high concentrations. Dose responses to prostanoids specific for EP prostanoid receptors were consistent with stimulating K secretion through EP<jats:sub>2<\/jats:sub>receptors, based on a rank order potency (from EC<jats:sub>50<\/jats:sub>values) of PGE<jats:sub>2<\/jats:sub>(1.9 nM) &gt; 11-deoxy-PGE<jats:sub>1<\/jats:sub>(8.3 nM) &gt; 19( R)-hydroxy-PGE<jats:sub>2<\/jats:sub>(13.9 nM) &gt; butaprost (67 nM) &gt; 17-phenyl-trinor-PGE<jats:sub>2<\/jats:sub>(307 nM) \u226b sulprostone (&gt;10 \u03bcM). An isoprostane, 8-iso-PGE<jats:sub>2<\/jats:sub>, stimulated K secretion with an EC<jats:sub>50<\/jats:sub>of 33 nM. Cl secretory response was stimulated by PGD<jats:sub>2<\/jats:sub>and BW-245C, a DP prostanoid receptor-specific agonist: BW-245C (15 nM) &gt; PGD<jats:sub>2<\/jats:sub>(30 nM) &gt; PGE<jats:sub>2<\/jats:sub>(203 nM). Agonists specific for FP, IP, and TP prostanoid receptors were ineffective in stimulating I<jats:sub>sc<\/jats:sub>and G<jats:sub>t<\/jats:sub>at concentrations &lt;1 \u03bcM. These results indicate that PGE<jats:sub>2<\/jats:sub>stimulated electrogenic K secretion through activation of EP<jats:sub>2<\/jats:sub>receptors and electrogenic KCl secretion through activation of DP receptors. Thus stimulation of Cl secretion in vivo would occur either via physiological concentrations of PGD<jats:sub>2<\/jats:sub>(&lt;100 nM) or pathophysiological concentrations of PGE<jats:sub>2<\/jats:sub>(&gt;100 nM) that could occur during inflammatory conditions.<\/jats:p>","DOI":"10.1152\/ajpgi.2001.281.4.g984","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T16:04:32Z","timestamp":1514131472000},"page":"G984-G996","source":"Crossref","is-referenced-by-count":28,"title":["Prostanoids stimulate K secretion and Cl secretion in guinea pig distal colon via distinct pathways"],"prefix":"10.1152","volume":"281","author":[{"given":"Dan R.","family":"Halm","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, Wright State University, Dayton, Ohio 45435"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan Troutman","family":"Halm","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Wright State University, Dayton, Ohio 45435"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(99)00164-X"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1985.sp015746"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-57083-4_3"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114086"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(97)01383-6"},{"key":"B6","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/0016-5085(80)90189-4","volume":"78","author":"Bukhave K","year":"1980","journal-title":"Gastroenterology"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1991.261.3.G443"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1990.259.1.G62"},{"key":"B10","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0031-6997(25)06784-5","volume":"46","author":"Coleman RA","year":"1994","journal-title":"Pharmacol Rev"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(83)90196-X"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-6980(98)00064-1"},{"key":"B13","first-page":"74","volume":"337","author":"Diener M","year":"1988","journal-title":"Naunyn Schmiedebergs Arch Pharmacol"},{"key":"B14","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1016\/S0022-3565(24)36950-2","volume":"283","author":"Elmhurst JL","year":"1997","journal-title":"J Pharmacol Exp Ther"},{"key":"B15","doi-asserted-by":"crossref","first-page":"483","DOI":"10.4049\/jimmunol.135.1.483","volume":"135","author":"Fox CC","year":"1985","journal-title":"J Immunol"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1007\/BF00410193"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1994.266.1.G132"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1991.260.6.G904"},{"key":"B20","first-page":"257","author":"Halm DR","year":"1991","journal-title":"Handbook of Physiology. Intestinal Absorption and Secretion."},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.2.C552"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.277.3.C501"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.4.C929"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1993.265.3.G423"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.262.6.C1392"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(85)90225-2"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1982.243.6.F519"},{"key":"B28","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S0022-3565(25)12649-9","volume":"255","author":"Hildebrand KR","year":"1990","journal-title":"J Pharmacol Exp Ther"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1995.268.2.G270"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1989.256.4.G673"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701367"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(88)90284-3"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(92)90083-6"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1007\/BF00704154"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1985.248.1.G103"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1997.272.3.G681"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1987.252.1.G120"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1999.79.4.1193"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-6980(00)00058-7"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1007\/BF01308038"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1993.264.5.G886"},{"key":"B42","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1016\/S0022-3565(25)12108-3","volume":"275","author":"Rangachari PK","year":"1995","journal-title":"J Pharmacol Exp Ther"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021398"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1996.271.4.G669"},{"key":"B45","first-page":"219","volume":"164","author":"Schultheiss G","year":"1998","journal-title":"Acta Physiol Scand"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(00)00008-4"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/0022-5193(77)90390-3"},{"key":"B48","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0022-3565(25)10303-0","volume":"264","author":"Traynor TR","year":"1993","journal-title":"J Pharmacol Exp Ther"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1995.269.2.R426"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1990.258.3.G432"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1988.255.1.G27"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112175"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.270.3.C832"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(99)00358-1"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1007\/BF01957352"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.2001.281.4.G984","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,29]],"date-time":"2025-06-29T05:14:40Z","timestamp":1751174080000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.2001.281.4.G984"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10,1]]},"references-count":53,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,10,1]]}},"alternative-id":["10.1152\/ajpgi.2001.281.4.G984"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.2001.281.4.g984","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10,1]]}}}