{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:22:52Z","timestamp":1778275372006,"version":"3.51.4"},"reference-count":43,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2001,12,1]]},"abstract":"<jats:p>Upregulation of the colonic H<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>- ATPase (cHKA) during hyperaldosteronism suggests that it functions in both K<jats:sup>+<\/jats:sup>conservation and electrogenic Na<jats:sup>+<\/jats:sup>absorption in the colon when Na<jats:sup>+<\/jats:sup>-conserving mechanisms are activated. To test this hypothesis, wild-type ( cHKA<jats:sup>+\/+<\/jats:sup>) and cHKA-deficient ( cHKA<jats:sup>\u2212\/\u2212<\/jats:sup>) mice were fed Na<jats:sup>+<\/jats:sup>-replete and Na<jats:sup>+<\/jats:sup>-restricted diets and their responses were analyzed. In both genotypes, Na<jats:sup>+<\/jats:sup>restriction led to reduced plasma Na<jats:sup>+<\/jats:sup>and increased serum aldosterone, and mRNAs for the epithelial Na<jats:sup>+<\/jats:sup>channel (ENaC) \u03b2- and \u03b3-subunits, channel-inducing factor, and cHKA were increased in distal colon. Relative to wild-type controls, cHKA<jats:sup>\u2212\/\u2212<\/jats:sup>mice on a Na<jats:sup>+<\/jats:sup>-replete diet had elevated fecal K<jats:sup>+<\/jats:sup>excretion. Dietary Na<jats:sup>+<\/jats:sup>restriction led to increased K<jats:sup>+<\/jats:sup>excretion in knockout but not in wild-type mice. The amiloride-sensitive, ENaC-mediated short-circuit current in distal colon was significantly reduced in knockout mice maintained on either the Na<jats:sup>+<\/jats:sup>-replete or Na<jats:sup>+<\/jats:sup>-restricted diet. These results demonstrate that cHKA plays an important role in K<jats:sup>+<\/jats:sup>conservation during dietary Na<jats:sup>+<\/jats:sup>restriction and suggest that cHKA-mediated K<jats:sup>+<\/jats:sup>recycling across the apical membrane is required for maximum electrogenic Na<jats:sup>+<\/jats:sup>absorption.<\/jats:p>","DOI":"10.1152\/ajpgi.2001.281.6.g1369","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T16:00:37Z","timestamp":1514131237000},"page":"G1369-G1377","source":"Crossref","is-referenced-by-count":27,"title":["Colonic H<sup>+<\/sup>-K<sup>+<\/sup>-ATPase in K<sup>+<\/sup>conservation and electrogenic Na<sup>+<\/sup>absorption during Na<sup>+<\/sup>restriction"],"prefix":"10.1152","volume":"281","author":[{"given":"Zachary","family":"Spicer","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry and Microbiology, The University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0524; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lane L.","family":"Clarke","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Veterinary Medicine and Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri 65211"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara R.","family":"Gawenis","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Veterinary Medicine and Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri 65211"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary E.","family":"Shull","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, Biochemistry and Microbiology, The University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0524; 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