{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T05:13:39Z","timestamp":1738559619722,"version":"3.35.0"},"reference-count":47,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:p>The excitatory amino acid glutamate plays an important role in the development of neuronal sensitization and the ionotropic N-methyl-d-aspartate receptor (NMDAR) is one of the major receptors involved. The objective of this study was to use a cat model of gastroesophageal reflux disease (GERD) to investigate the expression of the NR1 and NR2A subunits of NMDAR in the vagal and spinal afferent fibers innervating the esophagus. Two groups of cats (Acid-7D and PBS-7D) received 0.1 N HCl (pH 1.2) or 0.1 M PBS (pH 7.4) infusion in the esophagus (1 ml\/min for 30 min\/day for 7 days), respectively. NR1 splice variants (both NH<jats:sub>2<\/jats:sub>and COOH terminals) and NR2A in the thoracic dorsal root ganglia (DRGs), nodose ganglia (NGs), and esophagus were evaluated by RT-PCR, Western blot, and immunohistochemistry. Acid produced marked inflammation and a significant increase in eosinophil peroxidase and myeloperoxidase contents compared with PBS-infused esophagus. The NR1-4 splice variant gene exhibited a significant upregulation in DRGs and esophagus after acid infusion. In DRGs, NGs, and esophagus, acid infusion resulted in significant upregulation of NR1 and downregulation of NR2A subunit gene expression. A significant increase in NR1 polypeptide expression was observed in DRGs and NGs from Acid-7D compared with control. In conclusion, long-term acid infusion in the cat esophagus resulted in ulcerative esophagitis and differential expressions of NR1 and NR2A subunits. It is possible that these changes may in part contribute to esophageal hypersensitivity observed in reflux esophagitis.<\/jats:p>","DOI":"10.1152\/ajpgi.90419.2008","type":"journal-article","created":{"date-parts":[[2008,10,31]],"date-time":"2008-10-31T01:09:06Z","timestamp":1225415346000},"page":"G66-G77","source":"Crossref","is-referenced-by-count":17,"title":["Alterations in<i>N<\/i>-methyl-<scp>d<\/scp>-aspartate receptor subunits in primary sensory neurons following acid-induced esophagitis in cats"],"prefix":"10.1152","volume":"296","author":[{"given":"Banani","family":"Banerjee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bidyut K.","family":"Medda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyoti N.","family":"Sengupta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Shaker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.042688199"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2007.00947.x"},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Caudle RM, Perez FM, Del Valle-Pinero AY, Iadarola MJ.Spinal cord NR1 serine phosphorylation and NR2B subunit suppression following peripheral inflammation.Mol Pain1: 25, 2005.","DOI":"10.1186\/1744-8069-1-25"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(03)00700-6"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(92)90745-S"},{"key":"R5A","doi-asserted-by":"publisher","DOI":"10.1002\/neu.20084"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19980202)391:1<78::AID-CNE7>3.0.CO;2-O"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(00)00215-4"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.08.042"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(87)91487-9"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2007.02.063"},{"key":"R11","unstructured":"DeVault KR.Acid infusion does not affect intraesophageal balloon distention-induced sensory and pain thresholds.Am J Gastroenterol92: 947\u2013949, 1997."},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(87)90275-9"},{"key":"R13","doi-asserted-by":"crossref","unstructured":"Dingledine R, Borges K, Bowie D, Traynelis SF.The glutamate receptor ion channels.Pharmacol Rev51: 7\u201361, 1999.","DOI":"10.1016\/S0031-6997(24)01394-2"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70014-9"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(92)90245-7"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(89)90228-0"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2002.88.3.1512"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(93)91053-K"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2003.06.008"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(03)00750-X"},{"key":"R21","doi-asserted-by":"crossref","unstructured":"Ismail-Beigi F, Horton PF, Pope CE.Histological consequences of gastroesophageal reflux in man.Gastroenterology58: 163\u2013174, 1970.","DOI":"10.1016\/S0016-5085(70)80004-X"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(00)00357-2"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(02)00140-9"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1007\/BF01072525"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00097.2006"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.18.8383"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(95)11553-0"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(00)00419-X"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10202"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.24848"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.07.016"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90055-1"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2007.05.034"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-1-00621.x"},{"key":"R35","doi-asserted-by":"crossref","unstructured":"Peghini PL, Johnston BT, Leite LP, Castell DO.Mucosal acid exposure sensitizes a subset of normal subjects to intra-oesophageal balloon distension.Eur J Gastroenterol Hepatol8: 979\u2013983, 1996.","DOI":"10.1097\/00042737-199610000-00008"},{"key":"R36","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-12-05228.1993"},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(92)90039-E"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02758-6"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.281.5.G1196"},{"key":"R40","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199309000-00013"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-74806-9_15"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2007.07.008"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1038\/88404"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2003.11.047"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1016\/S1385-299X(99)00005-7"},{"key":"R47","doi-asserted-by":"crossref","unstructured":"Zhou Q, Caudle RM, Price DD, Del Valle-Pinero AY, Verne GN.Selective upregulation of NMDA-NR1 receptor expression in myenteric plexus after TNBS induced colitis in rats.Mol Pain2: 3, 2006.","DOI":"10.1186\/1744-8069-2-3"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.90419.2008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,2]],"date-time":"2025-02-02T12:52:15Z","timestamp":1738500735000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.90419.2008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.1152\/ajpgi.90419.2008"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.90419.2008","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"type":"print","value":"0193-1857"},{"type":"electronic","value":"1522-1547"}],"subject":[],"published":{"date-parts":[[2009,1]]}}}