{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T16:00:15Z","timestamp":1783180815318,"version":"3.54.6"},"reference-count":52,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:p>Myristoylated alanine-rich C kinase substrate (MARCKS) is known as a major cellular substrate for protein kinase C (PKC). MARCKS has been implicated in the regulation of brain development and postnatal survival, cellular migration and adhesion, as well as phagocytosis, endocytosis, and exocytosis. The involvement of MARCKS phosphorylation in secretory function has been reported in Ca<jats:sup>2+<\/jats:sup>-mediated exocytosis. In rat parotid acinar cells, the activation of \u03b2-adrenergic receptors provokes exocytotic amylase release via accumulation of intracellular cAMP levels. Here, we studied the involvement of MARCKS phosphorylation in the cAMP-dependent amylase release in rat parotid acinar cells. MARCKS protein was detected in rat parotid acinar cells by Western blotting. The \u03b2-adrenergic agonist isoproterenol (IPR) induced MARCKS phosphorylation in a time-dependent manner. Translocation of a part of phosphorylated MARCKS from the membrane to the cytosol and enhancement of MARCKS phosphorylation at the apical membrane site induced by IPR were observed by immunohistochemistry. H89, a cAMP-dependent protein kinase (PKA) inhibitor, inhibited the IPR-induced MARCKS phosphorylation. The PKC\u03b4 inhibitor rottlerin inhibited the IPR-induced MARCKS phosphorylation and amylase release. IPR activated PKC\u03b4, and the effects of IPR were inhibited by the PKA inhibitors. A MARCKS-related peptide partially inhibited the IPR-induced amylase release. These findings suggest that MARCKS phosphorylation via the activation of PKC\u03b4, which is downstream of PKA activation, is involved in the cAMP-dependent amylase release in parotid acinar cells.<\/jats:p>","DOI":"10.1152\/ajpgi.90536.2008","type":"journal-article","created":{"date-parts":[[2009,4,17]],"date-time":"2009-04-17T00:54:45Z","timestamp":1239929685000},"page":"G1382-G1390","source":"Crossref","is-referenced-by-count":20,"title":["Phosphorylation of myristoylated alanine-rich C kinase substrate is involved in the cAMP-dependent amylase release in parotid acinar cells"],"prefix":"10.1152","volume":"296","author":[{"given":"Keitaro","family":"Satoh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miwako","family":"Matsuki-Fukushima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Yu","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takanori","family":"Narita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junko","family":"Fujita-Yoshigaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Sugiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90546-O"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00630.2006"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1042\/bj3620001"},{"key":"R4","doi-asserted-by":"crossref","unstructured":"Aunis D, Bader M.The cytoskeleton as a barrier to exocytosis in secretory cells.J Exp Biol139: 253\u2013266, 1988.","DOI":"10.1242\/jeb.139.1.253"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(81)90056-X"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(55)01021-5"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1993.tb03303.x"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)02713-4"},{"key":"R10","doi-asserted-by":"crossref","unstructured":"Goodall AR, Turner NA, Walker JH, Ball SG, Vaughan PF.Activation of protein kinase C-\u03b1 and translocation of the myristoylated alanine-rich C kinase substrate correlate with phorbol ester-enhanced noradrenaline release from SH-SY5Y human neuroblastoma cells.J Neurochem68: 392\u2013401, 1997.","DOI":"10.1046\/j.1471-4159.1997.68010392.x"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.3.C692"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1038\/356618a0"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(89)90013-4"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.11.6903"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006328303451"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409431200"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105614200"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306960200"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2004.07.009"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403976200"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1042\/bj20021626"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.070318"},{"key":"R23","doi-asserted-by":"crossref","unstructured":"Putney JW Jr, MaKinney JS, Aub DL, Leslie BA.Phorbol ester-induced protein secretion in rat parotid gland.Mol Pharmacol26: 261\u2013266, 1984.","DOI":"10.1016\/S0026-895X(25)15081-5"},{"key":"R24","unstructured":"Quissell DO.Stimulus-exocytosis coupling mechanism in salivary gland cells. In:Biology of the Salivary Glands, edited by Dobrosielski-Vergona K. Boca Raton: CRC, 1993, p. 181\u2013200."},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1177\/10454411920030010701"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(99)00152-1"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006518200"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.017640"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00087.2004"},{"key":"R30","unstructured":"Segawa A, Yamashina S.Roles of microfilaments in exocytosis: a new hypothesis.Cell Struct Funct4: 531\u2013544, 1989."},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2004.07.021"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(88)90772-3"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a003271"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1038\/nm983"},{"key":"R35","doi-asserted-by":"crossref","unstructured":"Soltoff SP.Rottlerin is a mitochondrial uncoupler that decreases cellular ATP levels and indirectly blocks protein kinase C\u03b4 tyrosine phosphorylation.J Biol Chem276: 37986\u201337992, 2001.","DOI":"10.1074\/jbc.M105073200"},{"key":"R36","unstructured":"Spearman TN, Butcher FR.Rat parotid gland protein kinase activation: relationship to enzyme secretion.Mol Pharmacol21: 121\u2013127, 1982."},{"key":"R37","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.11.4012"},{"key":"R38","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604291200"},{"key":"R39","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.38.23424"},{"key":"R40","doi-asserted-by":"crossref","unstructured":"Takuma T, Ichida T.Catalytic subunit of protein kinase A induces amylase release from streptolysin O-permeabilized parotid acini.J Biol Chem269: 22124\u201322128, 1994.","DOI":"10.1016\/S0021-9258(17)31764-7"},{"key":"R41","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)80167-3"},{"key":"R42","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(96)00019-2"},{"key":"R43","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(90)90043-D"},{"key":"R44","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2005.10.007"},{"key":"R45","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(96)00308-5"},{"key":"R46","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(89)90140-4"},{"key":"R47","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)00193-0"},{"key":"R48","doi-asserted-by":"publisher","DOI":"10.1034\/j.1601-0825.2002.10815.x"},{"key":"R49","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M910298199"},{"key":"R50","unstructured":"Wulff K, D\u00f6ppen W.ATP: luminometric method. In:Methods of Enzymatic Analysis(3rd ed.), edited by Bergmeyer HU, Bergmeyer J, and Grabl M. Weinheim: VCH Verlagsgesellschaft, 1985, p. 357\u2013364."},{"key":"R51","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00474.2001"},{"key":"R52","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-1-00403.x"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpgi.90536.2008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,9]],"date-time":"2025-02-09T00:33:55Z","timestamp":1739061235000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpgi.90536.2008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6]]},"references-count":52,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.1152\/ajpgi.90536.2008"],"URL":"https:\/\/doi.org\/10.1152\/ajpgi.90536.2008","relation":{},"ISSN":["0193-1857","1522-1547"],"issn-type":[{"value":"0193-1857","type":"print"},{"value":"1522-1547","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6]]}}}