{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:46:55Z","timestamp":1787017615776,"version":"build-2736575974"},"reference-count":45,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Gastrointestinal and Liver Physiology"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:p>\n                    Pancreatic ductal epithelium produces a HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    -rich fluid. HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    transport across ductal apical membranes has been proposed to be mediated by both SLC26-mediated Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    \/HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    exchange and CFTR-mediated HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    conductance, with proportional contributions determined in part by axial changes in gene expression and luminal anion composition. In this study we investigated the characteristics of apical Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    \/HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    exchange and its functional interaction with Cftr activity in isolated interlobular ducts of guinea pig pancreas. BCECF-loaded epithelial cells of luminally microperfused ducts were alkalinized by acetate prepulse or by luminal Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    removal in the presence of HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    -CO\n                    <jats:sub>2<\/jats:sub>\n                    . Intracellular pH recovery upon luminal Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    restoration (nominal Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    \/HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    exchange) in cAMP-stimulated ducts was largely inhibited by luminal dihydro-DIDS (H\n                    <jats:sub>2<\/jats:sub>\n                    DIDS), accelerated by luminal CFTR inhibitor inh-172 (CFTRinh-172), and was insensitive to elevated bath K\n                    <jats:sup>+<\/jats:sup>\n                    concentration. Luminal introduction of CFTRinh-172 into sealed duct lumens containing BCECF-dextran in HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    -free, Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    -rich solution enhanced cAMP-stimulated HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    secretion, as calculated from changes in luminal pH and volume. Luminal Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    removal produced, after a transient small depolarization, sustained cell hyperpolarization of \u223c15 mV consistent with electrogenic Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    \/HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    exchange. The hyperpolarization was inhibited by H\n                    <jats:sub>2<\/jats:sub>\n                    DIDS and potentiated by CFTRinh-172. Interlobular ducts expressed mRNAs encoding CFTR, Slc26a6, and Slc26a3, as detected by RT-PCR. Thus Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    -dependent apical HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    secretion in pancreatic duct is mediated predominantly by an Slc26a6-like Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    \/HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    exchanger and is accelerated by inhibition of CFTR. This study demonstrates functional coupling between Cftr and Slc26a6-like Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    \/HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    exchange activity in apical membrane of guinea pig pancreatic interlobular duct.\n                  <\/jats:p>","DOI":"10.1152\/ajpgi.90697.2008","type":"journal-article","created":{"date-parts":[[2009,4,2]],"date-time":"2009-04-02T21:41:44Z","timestamp":1238708504000},"page":"G1307-G1317","source":"Crossref","is-referenced-by-count":57,"title":["Functional coupling of apical Cl\n                    <sup>\u2212<\/sup>\n                    \/HCO\n                    <sub>3<\/sub>\n                    <sup>\u2212<\/sup>\n                    exchange with CFTR in stimulated HCO\n                    <sub>3<\/sub>\n                    <sup>\u2212<\/sup>\n                    secretion by guinea pig interlobular pancreatic duct"],"prefix":"10.1152","volume":"296","author":[{"given":"A. K.","family":"Stewart","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Nakakuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. L.","family":"Alper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Ishiguro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","unstructured":"Argent B\n                      , Gray M, Steward M, Case M.\n                      Cell Physiology of Pancreatic Ducts. In:\n                      Physiology of the Gastrointestinal Tract\n                      , edited by Johnson LR, Barrett KE, Ghishan FK, Merchant JL, Said HM, Wood JD. Burlington, MA: Elsevier Academic, 2006, p. 1371\u20131396."},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20080029"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411703200"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.039818"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1038\/35065099"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.143222"},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00037.2007"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2000.15.S.S12"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1989.257.2.C240"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.281.5.G1301"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00574.2002"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00430.2003"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00286.2006"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0551m.x"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.012490"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.00305.x"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.407bh.x"},{"key":"R18","unstructured":"Ishiguro H\n                      , Steward M, Naruse S.\n                      Cystic fibrosis transmembrane conductance regulator and SLC26 transporters in HCO3\n                      \u2212\n                      secretion by pancreatic duct cells.\n                      Sheng Li Xue Bao\n                      59\n                      : 465\u2013476, 2007."},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021582"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.20028631"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021583"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf580"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1115"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-006-0049-6"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.21.14670"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.6.3414"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205932200"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.32.22855"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1007\/BF00581647"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1007\/BF00582376"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1097\/00006676-198904000-00009"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v12.i6.885"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21220"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313323200"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200509392"},{"key":"R36","doi-asserted-by":"crossref","unstructured":"Sohma Y\n                      , Gray MA, Imai Y, Argent HCO\n                      \n                        3\n                      \n                      \n                        \u2212\n                      \n                      BE.\n                      transport in a mathematical model of the pancreatic ductal epithelium.\n                      J Membr Biol\n                      176\n                      : 77\u2013100, 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       secretion by stimulating the transporters pNBC1 and CFTR in the murine pancreatic duct.\n                      J Clin Invest\n                      119\n                      : 193\u2013202, 2009.","DOI":"10.1172\/JCI36983"}],"container-title":["American Journal of Physiology-Gastrointestinal and Liver 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