{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:48:05Z","timestamp":1792712885280,"version":"4.5.0"},"reference-count":35,"publisher":"American Physiological Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2016,5,1]]},"abstract":"<jats:p> Aging is associated with vascular dysfunction that impairs tissue perfusion, physical activity, and the quality of life. Calcium signaling in endothelial cells (ECs) is integral to vasomotor control, exemplified by localized Ca<jats:sup>2+<\/jats:sup> signals within EC projections through holes in the internal elastic lamina (IEL). Within these microdomains, endothelium-derived hyperpolarization is integral to smooth muscle cell (SMC) relaxation via coupling through myoendothelial gap junctions. However, the effects of aging on local EC Ca<jats:sup>2+<\/jats:sup> signals (and thereby signaling between ECs and SMCs) remain unclear, and these events have not been investigated in vivo. Furthermore, it is unknown whether aging affects either the number or the size of IEL holes. In the present study, we tested the hypothesis that local EC Ca<jats:sup>2+<\/jats:sup> signaling is impaired with advanced age along with a reduction in IEL holes. In anesthetized mice expressing a Ca<jats:sup>2+<\/jats:sup>-sensitive fluorescent protein (GCaMP2) selectively in ECs, our findings illustrate that for mesenteric arteries controlling splanchnic blood flow the frequency of spontaneous local Ca<jats:sup>2+<\/jats:sup> signals in ECs was reduced by \u223c85% in old (24\u201326 mo) vs. young (3\u20136 mo) animals. At the same time, the number (and total area) of holes per square millimeter of IEL was reduced by \u223c40%. We suggest that diminished signaling between ECs and SMCs contributes to dysfunction of resistance arteries with advanced age. <\/jats:p><jats:p> Listen to this article's corresponding podcast at http:\/\/ajpheart.podbean.com\/e\/aging-impairs-endothelial-ca2-signaling\/ . <\/jats:p>","DOI":"10.1152\/ajpheart.00038.2016","type":"journal-article","created":{"date-parts":[[2016,3,4]],"date-time":"2016-03-04T22:39:51Z","timestamp":1457131191000},"page":"H1091-H1096","source":"Crossref","is-referenced-by-count":33,"title":["Advanced age decreases local calcium signaling in endothelium of mouse mesenteric arteries in vivo"],"prefix":"10.1152","volume":"310","author":[{"given":"Erika M.","family":"Boerman","sequence":"first","affiliation":[{"name":"Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, Missouri; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse E.","family":"Everhart","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, Missouri; 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