{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T20:53:07Z","timestamp":1772657587940,"version":"3.50.1"},"reference-count":38,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2002,9,1]]},"abstract":"<jats:p> A phospholipid vesicle encapsulating hemoglobin (Hb vesicle, HbV) has been developed to provide O<jats:sub>2<\/jats:sub>-carrying capacity to plasma expanders. Its ability to restore systemic and microcirculatory conditions after hemorrhagic shock was evaluated in the dorsal skinfold window preparation of conscious hamsters. The HbV was suspended in 8% human serum albumin (HSA) at Hb concentrations of 3.8 g\/dl [HbV(3.8)\/HSA] and 7.6 g\/dl [HbV(7.6)\/HSA]. Shock was induced by 50% blood withdrawal, and mean arterial pressure (MAP) at 40 mmHg was maintained for 1 h by the additional blood withdrawal. The hamsters receiving either HbV(3.8)\/HSA or HbV(7.6)\/HSA suspensions restored MAP to 93 \u00b1 14 and 93 \u00b1 10 mmHg, respectively, similar with those receiving the shed blood (98 \u00b1 13 mmHg), which were significantly higher by comparison with resuscitation with HSA alone (62 \u00b1 12 mmHg). Only the HSA group tended to maintain hyperventilation and negative base excess after the resuscitation. Subcutaneous microvascular blood flow reduced to \u223c10\u201320% of baseline during shock, and reinfusion of shed blood restored blood flow to \u223c60\u201380% of baseline, an effect primarily due to the sustained constriction of small arteries A<jats:sub>0<\/jats:sub> (diameter 143 \u00b1 29 \u03bcm). The HbV(3.8)\/HSA group had significantly better microvascular blood flow recovery and nonsignificantly better tissue oxygenation than of the HSA group. The recovery of base excess and improved tissue oxygenation appears to be primarily due to the increased oxygen-carrying capacity of HbV fluid resuscitation. <\/jats:p>","DOI":"10.1152\/ajpheart.00080.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T15:03:45Z","timestamp":1425395025000},"page":"H1191-H1199","source":"Crossref","is-referenced-by-count":51,"title":["Systemic and microvascular responses to hemorrhagic  shock and resuscitation with Hb vesicles"],"prefix":"10.1152","volume":"283","author":[{"given":"Hiromi","family":"Sakai","sequence":"first","affiliation":[{"name":"Advanced Research Institute for Science and Engineering, Waseda University, Tokyo 169-8555, Japan; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinji","family":"Takeoka","sequence":"additional","affiliation":[{"name":"Advanced Research Institute for Science and Engineering, Waseda University, Tokyo 169-8555, Japan; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reto","family":"Wettstein","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California, San Diego, La Jolla, California 92093-0412"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy G.","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California, San Diego, La Jolla, California 92093-0412"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos","family":"Intaglietta","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California, San Diego, La Jolla, California 92093-0412"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eishun","family":"Tsuchida","sequence":"additional","affiliation":[{"name":"Advanced Research Institute for Science and Engineering, Waseda University, Tokyo 169-8555, Japan; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1998.274.4.F744"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-199703000-00007"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-198704000-00008"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1324"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1993.74.4.1769"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(96)00110-1"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(73)90042-3"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(75)90004-7"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199611000-00017"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1997.272.1.H525"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.268.2.H802"},{"key":"B13","first-page":"83","volume":"32","author":"Kreimeier U","year":"1990","journal-title":"Circ Shock"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.23249"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(78)90009-2"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199806000-00030"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-4622(01)00194-6"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1097\/00000539-200107000-00029"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2143(98)90045-2"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-200011000-00020"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1979.46.4.806"},{"key":"B22","first-page":"115","volume":"40","author":"Pascual JMS","year":"1993","journal-title":"Circ Shock"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.23.10976"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.276.2.H563"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1006\/mvre.2000.2240"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.279.3.H908"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)61783-X"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1021\/bc960069p"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1021\/bc990173h"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199804)40:1<66::AID-JBM8>3.0.CO;2-P"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.276.2.H553"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1991.70.1.169"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199509000-00015"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1993.265.4.H1434"},{"key":"B37","first-page":"229","volume":"38","author":"Tsai AG","year":"2001","journal-title":"Biorheology"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.6590"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.275.6.H2170"},{"key":"B42","first-page":"1153","volume":"51","author":"Vaslef SN","year":"2001","journal-title":"J Trauma"},{"key":"B43","first-page":"18","volume":"5","author":"Yoshizu A","year":"1997","journal-title":"Artif Blood"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00080.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T15:01:55Z","timestamp":1567954915000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00080.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9,1]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,9,1]]}},"alternative-id":["10.1152\/ajpheart.00080.2002"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00080.2002","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,9,1]]}}}