{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:35:31Z","timestamp":1791275731000,"version":"4.1.0"},"reference-count":54,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2002,11,1]]},"abstract":"<jats:p>The basolateral Na<jats:sup>+<\/jats:sup>-K<jats:sup>+<\/jats:sup>-2Cl<jats:sup>\u2212<\/jats:sup>cotransporter (NKCC1) functions in the maintenance of cellular electrolyte and volume homeostasis. NKCC1-deficient ( Nkcc1<jats:sup>\u2212\/\u2212<\/jats:sup>) mice were used to examine its role in cardiac function and in the maintenance of blood pressure and vascular tone. Tail-cuff measurements demonstrated that awake Nkcc1<jats:sup>\u2212\/\u2212<\/jats:sup>mice had significantly lower systolic blood pressure than wild-type ( Nkcc1<jats:sup>+\/+<\/jats:sup>) mice (114.5 \u00b1 2.2 and 131.8 \u00b1 2.5 mmHg, respectively). Serum aldosterone levels were normal, indicating that extracellular fluid-volume homeostasis was not impaired. Studies using pressure transducers in the femoral artery and left ventricle showed that anesthetized Nkcc1<jats:sup>\u2212\/\u2212<\/jats:sup>mice have decreased mean arterial pressure and left ventricular pressure, whereas myocardial contraction parameters were not significantly different from those of Nkcc1<jats:sup>+\/+<\/jats:sup>mice. When stimulated with phenylephrine, aortic smooth muscle from Nkcc1<jats:sup>+\/+<\/jats:sup>and Nkcc1<jats:sup>\u2212\/\u2212<\/jats:sup>mice exhibited no significant differences in maximum contractility and only moderate dose-response shifts. In phasic portal vein smooth muscle from Nkcc1<jats:sup>\u2212\/\u2212<\/jats:sup>mice, however, a sharp reduction in mechanical force was noted. These results indicate that NKCC1 can be important for the maintenance of normal blood pressure and vascular tone.<\/jats:p>","DOI":"10.1152\/ajpheart.00083.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:04:15Z","timestamp":1425413055000},"page":"H1846-H1855","source":"Crossref","is-referenced-by-count":99,"title":["Decreased blood pressure and vascular smooth muscle tone in mice lacking basolateral Na<sup>+<\/sup>-K<sup>+<\/sup>-2Cl<sup>\u2212<\/sup>cotransporter"],"prefix":"10.1152","volume":"283","author":[{"given":"Jamie W.","family":"Meyer","sequence":"first","affiliation":[{"name":"Departments of Molecular Genetics, Biochemistry, and Microbiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Flagella","sequence":"additional","affiliation":[{"name":"Departments of Molecular Genetics, Biochemistry, and Microbiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy 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