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Male Fischer 344 rats were divided into five groups ( n = 6\u20137): sedentary (Sed); exercised 2 days on a treadmill at 20 m\/min, 6\u00b0 grade, for 60 min (Run); sedentary, perfused with 100 \u03bcM N<jats:sup>\u03c9<\/jats:sup>-nitro-l-arginine methyl ester hydrochloride (l-NAME) to inhibit NOS (Sed\/L-N); exercised, perfused with l-NAME (Run\/L-N); and exercised in a 4\u00b0C environment, perfused with l-NAME (CRun\/L-N). Twenty-four hours following exercise, isolated, perfused working hearts were subjected to 22.5 min of global ischemia plus 30 min of normoxic reperfusion. Left ventricle contents of several putative preconditioning mediators were determined. Postischemic recovery of cardiac output times systolic pressure was better in Run than Sed (78.4 vs. 50.2% of preischemia, P &lt; 0.05). Inhibition of NOS did not abrogate the improved recovery in the exercise groups or alter recovery in Sed. All exercise groups also displayed improved myocardial efficiency (cardiac output times systolic pressure\/oxygen consumption) postischemia and less lactate dehydrogenase release ( P &lt; 0.05). l-NAME appeared to lower lactate dehydrogenase release independent of exercise. The only change in antioxidant enzyme activity was a decrease in manganese superoxide dismutase in CRun\/L-N ( P &lt; 0.05). Heat shock protein 72 expression increased only in Run and Run\/L-N and endothelial NOS only in CRun\/L-N ( P &lt; 0.05). Acute exercise-induced preconditioning of the Fischer 344 rat heart is not mediated by NOS and does not require increases in heat shock protein 72 or antioxidant enzymes.<\/jats:p>","DOI":"10.1152\/ajpheart.00094.2006","type":"journal-article","created":{"date-parts":[[2006,9,2]],"date-time":"2006-09-02T00:49:09Z","timestamp":1157158149000},"page":"H601-H607","source":"Crossref","is-referenced-by-count":27,"title":["Improved postischemic function following acute exercise is not mediated by nitric oxide synthase in the rat heart"],"prefix":"10.1152","volume":"292","author":[{"given":"Ryan P.","family":"Taylor","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marissa E.","family":"Olsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph W.","family":"Starnes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(02)00812-X"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703942"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1042\/cs1020435"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2003.10.025"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(02)00675-2"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(01)00472-2"},{"key":"R7","doi-asserted-by":"crossref","unstructured":"Bergmeyer H, Bernt E, and Hess B.Methods of Enzymatic Analysis.New York: Academic, 1965.","DOI":"10.1016\/B978-0-12-395630-9.50008-6"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1023\/A:1024251607923"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2001.1462"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000137171.97172.d7"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.81.6.1094"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1999.79.2.609"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1992.263.3.H804"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.095729"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.081323"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1998.0789"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(77)90662-5"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2001.91.5.2205"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(01)00306-6"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.02.008"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.549bh.x"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.281.3.H1346"},{"key":"R23","doi-asserted-by":"crossref","unstructured":"Han H, Kaiser R, Hu K, Laser M, Ertl G, and Bauersachs J.Selective modulation of endogenous nitric oxide formation in ischemia\/reperfusion injury in isolated rat hearts\u2013effects on regional myocardial flow and enzyme release.Basic Res Cardiol98: 165\u2013174, 2003.","DOI":"10.1007\/s00395-003-0408-9"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00726.2002"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.280.5.H2271"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01012.2001"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00236.2002"},{"key":"R28","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(02)00268-7"},{"key":"R29","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00396.2005"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.23.2742"},{"key":"R31","doi-asserted-by":"publisher","DOI":"10.1161\/hc5001.100039"},{"key":"R32","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"R33","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00920.2003"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01208.2003"},{"key":"R35","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.269.1.H320"},{"key":"R36","doi-asserted-by":"crossref","unstructured":"Lowry OH, Rosebrough NJ, Farr AL, and Randall RJ.Protein measurement with the Folin phenol reagent.J Biol Chem193: 265\u2013275, 1951.","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"R37","doi-asserted-by":"crossref","unstructured":"McCord JMand Fridovich I.Superoxide dismutase. 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