{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:57:00Z","timestamp":1790492220360,"version":"4.1.0"},"reference-count":41,"publisher":"American Physiological Society","issue":"2","funder":[{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute (NHBLI)","doi-asserted-by":"publisher","award":["HL093140"],"award-info":[{"award-number":["HL093140"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS)","doi-asserted-by":"publisher","award":["NS100459"],"award-info":[{"award-number":["NS100459"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2019,8,1]]},"abstract":"<jats:p> Capillary derecruitment distal to a coronary stenosis is implicated as the mechanism of reversible perfusion defect and potential myocardial ischemia during coronary hyperemia; however, the underlying mechanisms are not defined. We tested whether pericyte constriction underlies capillary derecruitment during hyperemia under conditions of stenosis. In vivo two-photon microscopy (2PM) and optical microangiography (OMAG) were used to measure hyperemia-induced changes in capillary diameter and perfusion in wild-type and pericyte-depleted mice with femoral artery stenosis. OMAG demonstrated that hyperemic challenge under stenosis produced capillary derecruitment associated with decreased RBC flux. 2PM demonstrated that hyperemia under control conditions induces 26\u2009\u00b1\u20095% of capillaries to dilate and 19\u2009\u00b1\u20093% to constrict. After stenosis, the proportion of capillaries dilating to hyperemia decreased to 14\u2009\u00b1\u20094% ( P = 0.05), whereas proportion of constricting capillaries increased to 32\u2009\u00b1\u20094% ( P = 0.05). Hyperemia-induced changes in capillary diameter occurred preferentially in capillary segments invested with pericytes. In a transgenic mouse model featuring partial pericyte depletion, only 14\u2009\u00b1\u20093% of capillaries constricted to hyperemic challenge after stenosis, a significant reduction from 33\u2009\u00b1\u20094% in wild-type littermate controls ( P = 0.04). These results provide for the first time direct visualization of hyperemia-induced capillary derecruitment distal to arterial stenosis and demonstrate that pericyte constriction underlies this phenomenon in vivo. These results could have important therapeutic implications in the treatment of exercise-induced ischemia. <\/jats:p><jats:p> NEW &amp; NOTEWORTHY In the setting of coronary arterial stenosis, hyperemia produces a reversible perfusion defect resulting from capillary derecruitment that is believed to underlie cardiac ischemia under hyperemic conditions. We use optical microangiography and in vivo two-photon microscopy to visualize capillary derecruitment distal to a femoral arterial stenosis with cellular resolution. We demonstrate that capillary constriction in response to hyperemia in the setting of stenosis is dependent on pericytes, contractile mural cells investing the microcirculation. <\/jats:p>","DOI":"10.1152\/ajpheart.00097.2019","type":"journal-article","created":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T13:47:51Z","timestamp":1558705671000},"page":"H255-H263","source":"Crossref","is-referenced-by-count":31,"title":["Pericyte constriction underlies capillary derecruitment during hyperemia in the setting of arterial stenosis"],"prefix":"10.1152","volume":"317","author":[{"given":"Carmen","family":"Methner","sequence":"first","affiliation":[{"name":"Knight Cardiovascular Institute, Oregon Health &amp; Science University, Portland, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anusha","family":"Mishra","sequence":"additional","affiliation":[{"name":"Knight Cardiovascular Institute, Oregon Health &amp; Science University, Portland, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsti","family":"Golgotiu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine, Oregon Health &amp; Science University, Portland, Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuandong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of Washington, Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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