{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T15:17:18Z","timestamp":1772032638917,"version":"3.50.1"},"reference-count":46,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:p>Extracellular ATP is known to augment cardiac contractility by increasing intracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) in cardiomyocytes; however, the status of ATP-mediated Ca<jats:sup>2+<\/jats:sup>mobilization in hearts undergoing ischemia-reperfusion (I\/R) has not been examined previously. In this study, therefore, isolated rat hearts were subjected to 10\u201330 min of global ischemia and 30 min of reperfusion, and the effect of extracellular ATP on [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>was measured in purified cardiomyocytes by fura-2 microfluorometry. Reperfusion for 30 min of 20-min ischemic hearts, unlike 10-min ischemic hearts, revealed a partial depression in cardiac function and ATP-induced increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>; no changes in basal [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>were evident in 10- or 20-min I\/R preparations. On the other hand, reperfusion of 30-min ischemic hearts for 5, 15, or 30 min showed a marked depression in both cardiac function and ATP-induced increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>and a dramatic increase in basal [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. The positive inotropic effect of extracellular ATP was attenuated, and the maximal binding characteristics of<jats:sup>35<\/jats:sup>S-labeled adenosine 5\u2032-[\u03b3-thio]triphosphate with crude membranes from hearts undergoing I\/R was decreased. ATP-induced increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>in cardiomyocytes was depressed by verapamil and Cibacron Blue in both control and I\/R hearts; however, this response in I\/R hearts, unlike control hearts, was not affected by ryanodine. I\/R-induced alterations in cardiac function and ATP-induced increase in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>were attenuated by treatment with an antioxidant mixture and by ischemic preconditioning. The observed changes due to I\/R were simulated in hearts perfused with H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. The results suggest an impairment of extracellular ATP-induced Ca<jats:sup>2+<\/jats:sup>mobilization in I\/R hearts, and this defect appears to be mediated through oxidative stress.<\/jats:p>","DOI":"10.1152\/ajpheart.00101.2005","type":"journal-article","created":{"date-parts":[[2005,4,9]],"date-time":"2005-04-09T00:25:28Z","timestamp":1113006328000},"page":"H614-H623","source":"Crossref","is-referenced-by-count":18,"title":["Attenuation of extracellular ATP response in cardiomyocytes isolated from hearts subjected to ischemia-reperfusion"],"prefix":"10.1152","volume":"289","author":[{"given":"Harjot K.","family":"Saini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vijayan","family":"Elimban","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naranjan S.","family":"Dhalla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.281.4.H1508"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9861(02)00733-6"},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Bers DM.Ryanodine and calcium content of cardiac SR assessed by caffeine and rapid cooling contractures.Am J Physiol Cell Physiol253: C408\u2013C415, 1987.","DOI":"10.1152\/ajpcell.1987.253.3.C408"},{"key":"R4","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9149(99)00316-1"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.58.3.319"},{"key":"R6","unstructured":"Burnstock G.Noradrenaline and ATP: cotransmitters and neuromodulators.J Physiol Pharmacol46: 365\u2013384, 1995."},{"key":"R7","doi-asserted-by":"publisher","DOI":"10.1016\/S0188-4409(03)00004-3"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(99)00222-3"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp018929"},{"key":"R10","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1989.257.4.C750"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(00)00078-X"},{"key":"R12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.262.3.C664"},{"key":"R13","doi-asserted-by":"crossref","unstructured":"Downey JM, Omar B, Ooiwa H, and McCord J.Superoxide dismutase therapy for myocardial ischemia.Free Radic Res Commun12\u201313: 703\u2013720, 1991.","DOI":"10.3109\/10715769109145850"},{"key":"R14","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.76.6.1036"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-198809000-00003"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1161\/hh2101.098547"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1042\/bj3360513"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.3.C766"},{"key":"R19","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.66.5.1255"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(94)90089-2"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.268.1.H100"},{"key":"R22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1996.tb33500.x"},{"key":"R23","doi-asserted-by":"crossref","unstructured":"Nayler WG.Basic mechanisms involved in the protection of the ischaemic myocardium. 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