{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:48:48Z","timestamp":1760215728296},"reference-count":36,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:p>The \u03b1<jats:sub>2<\/jats:sub>-isoform of Na,K-ATPase (\u03b1<jats:sub>2<\/jats:sub>) is thought to play a role in blood pressure regulation, but the specific cell type(s) involved have not been identified. Therefore, it is important to study the role of the \u03b1<jats:sub>2<\/jats:sub>in individual cell types in the cardiovascular system. The present study demonstrates the role of vascular smooth muscle \u03b1<jats:sub>2<\/jats:sub>in the regulation of cardiovascular hemodynamics. To accomplish this, we developed a mouse model utilizing the Cre\/LoxP system to generate a cell type-specific knockout of the \u03b1<jats:sub>2<\/jats:sub>in vascular smooth muscle cells using the SM22\u03b1 Cre. We achieved a 90% reduction in the \u03b1<jats:sub>2<\/jats:sub>-expression in heart and vascular smooth muscle in the knockout mice. Interestingly, tail-cuff blood pressure analysis reveals that basal systolic blood pressure is unaffected by the knockout of \u03b1<jats:sub>2<\/jats:sub>in the knockout mice. However, knockout mice do fail to develop ACTH-induced hypertension, as seen in wild-type mice, following 5 days of treatment with ACTH (Cortrosyn; wild type = 119.0 \u00b1 6.8 mmHg; knockout = 103.0 \u00b1 2.0 mmHg). These results demonstrate that \u03b1<jats:sub>2<\/jats:sub>-expression in heart and vascular smooth muscle is not essential for regulation of basal systolic blood pressure, but \u03b1<jats:sub>2<\/jats:sub>is critical for blood pressure regulation under chronic stress such as ACTH-induced hypertension.<\/jats:p>","DOI":"10.1152\/ajpheart.00121.2011","type":"journal-article","created":{"date-parts":[[2011,8,22]],"date-time":"2011-08-22T21:44:32Z","timestamp":1314049472000},"page":"H1396-H1404","source":"Crossref","is-referenced-by-count":24,"title":["Knockout of the Na,K-ATPase \u03b1<sub>2<\/sub>-isoform in the cardiovascular system does not alter basal blood pressure but prevents ACTH-induced hypertension"],"prefix":"10.1152","volume":"301","author":[{"given":"Tara N.","family":"Rindler","sequence":"first","affiliation":[{"name":"Departments of 1Molecular Genetics, Biochemistry, and Microbiology 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