{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:09:42Z","timestamp":1787497782843,"version":"build-2736575974"},"reference-count":63,"publisher":"American Physiological Society","issue":"4","funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["19POST34380255"],"award-info":[{"award-number":["19POST34380255"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["FIUF 2400160"],"award-info":[{"award-number":["FIUF 2400160"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["1R01HL160740"],"award-info":[{"award-number":["1R01HL160740"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["K12HL143959"],"award-info":[{"award-number":["K12HL143959"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007681","name":"Florida International University","doi-asserted-by":"publisher","award":["Dissertation Year Fellowship"],"award-info":[{"award-number":["Dissertation Year Fellowship"]}],"id":[{"id":"10.13039\/100007681","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Florida Heart Research Foundation","award":["Stop Heart Disease Researcher of the Year Award"],"award-info":[{"award-number":["Stop Heart Disease Researcher of the Year Award"]}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2023,4,1]]},"abstract":"<jats:p>Here, we investigate the potential of epidermal growth factor receptor (EGFR) inhibition to prevent vascular calcification, a leading indicator of and contributor to cardiovascular morbidity and mortality. EGFR interacts and affects the trafficking of the plasma membrane scaffolding protein caveolin-1. Previous studies reported a key role for caveolin-1 in the development of specialized extracellular vesicles that mediate vascular calcification; however, no role of EGFR has been reported. We demonstrated that EGFR inhibition modulates caveolin-1 trafficking and hinders calcifying extracellular vesicle formation, which prevents vascular calcification. Given that EGFR inhibitors are clinically approved for other indications, this may represent a novel therapeutic strategy for vascular calcification.<\/jats:p>","DOI":"10.1152\/ajpheart.00280.2022","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T13:19:56Z","timestamp":1677244796000},"page":"H553-H570","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":25,"title":["Epidermal growth factor receptor inhibition prevents vascular calcifying extracellular vesicle biogenesis"],"prefix":"10.1152","volume":"324","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1176-1628","authenticated-orcid":false,"given":"Amirala","family":"Bakhshian Nik","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Florida International University, Miami, Florida, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2370-0849","authenticated-orcid":false,"given":"Hooi Hooi","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Florida International University, Miami, Florida, United States"},{"name":"Department of Human and Molecular Genetics, Herbert Wertheim College of Medicine, Florida International University, Miami, Florida, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie K.","family":"Ashbrook","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Florida International University, Miami, Florida, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of BioHealth Informatics, Luddy School of Informatics,         Computing, and Engineering, Indiana University, Indianapolis, Indiana, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3564-2380","authenticated-orcid":false,"given":"Francesco","family":"Iacoviello","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, University College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Shearing","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, University College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4889-8190","authenticated-orcid":false,"given":"Sergio","family":"Bertazzo","sequence":"additional","affiliation":[{"name":"Department of Medical Physics and Biomedical Engineering, University College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deniel","family":"Mero","sequence":"additional","affiliation":[{"name":"Dock Therapeutics, Inc., Middletown, Delaware, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bohdan B.","family":"Khomtchouk","sequence":"additional","affiliation":[{"name":"Department of BioHealth Informatics, Luddy School of Informatics,         Computing, and Engineering, Indiana University, Indianapolis, Indiana, United States"},{"name":"Krannert Cardiovascular Research Center, Indiana University School of Medicine, Indianapolis, Indiana, United States"},{"name":"Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8383-4435","authenticated-orcid":false,"given":"Joshua D.","family":"Hutcheson","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Florida International University, Miami, Florida, United States"},{"name":"Biomolecular Sciences Institute, Florida International University, Miami, Florida, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.116.306717"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1159\/000350764"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2017.00078"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ekir.2018.07.011"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfg414"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2007080854"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/j.carpath.2015.03.002"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.112.300128"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2015.05.013"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-46725-8"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1172\/JCI80851"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.11.024"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000129178.56294.17"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R200020200"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-7440-8"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2009.2908"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2007.03.005"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.5732\/cjc.010.10542"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2018.8533"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-11331-9"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1038\/srep45917"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2012.12.013"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02351-6"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1159\/000347031"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.06.016"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1038\/nmat4519"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1811725118"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00786.2014"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.03.011"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1038\/srep39604"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.bonr.2020.100261"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.3390\/jcdd9060168"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2010.08.023"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.15555.1"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0175-2"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/dyx034"},{"key":"B37","volume-title":"R: A Language and Environment for Statistical Computing","author":"R CoreTeam.","year":"2021"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4477"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.12659\/msmbr.901142"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-009-0832-8"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00593.2003"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1113\/JP271339"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2017.08.036"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.11.7481"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207911"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-7-69"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1112129109"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.080604"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.305012"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0190985"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.070601"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.598581"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2009.07.001"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.16544.1"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1002\/sim.3034"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-84851-0"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCGEN.118.002426"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baaa115"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.34408"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbz119"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.119.044195"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2011.648617"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.119.311994"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00280.2022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T07:51:33Z","timestamp":1679557893000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpheart.00280.2022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,1]]},"references-count":63,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,4,1]]}},"alternative-id":["10.1152\/ajpheart.00280.2022"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00280.2022","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,1]]},"assertion":[{"value":"2022-06-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-02","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}