{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T20:41:57Z","timestamp":1759092117511},"reference-count":32,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p> Cardiac ventricular myocytes possess an extensive t-tubular system that facilitates the propagation of membrane potential across the cell body. It is well established that ionic currents at the restricted t-tubular space may lead to significant changes in ion concentrations, which, in turn, may affect t-tubular membrane potential. In this study, we used the whole cell patch-clamp technique to study accumulation and depletion of t-tubular potassium by measuring inward rectifier potassium tail currents ( I<jats:sub>K1,tail<\/jats:sub>), and inward rectifier potassium current ( I<jats:sub>K1<\/jats:sub>) \u201cinactivation\u201d. At room temperatures and in the absence of Mg<jats:sup>2+<\/jats:sup> ions in pipette solution, the amplitude of I<jats:sub>K1,tail<\/jats:sub> measured \u223c10 min after the establishment of whole cell configuration was reduced by \u223c18%, but declined nearly twofold in the presence of 1 mM cyanide. At \u223c35\u00b0C I<jats:sub>K1,tail<\/jats:sub> was essentially preserved in intact cells, but its amplitude declined by \u223c85% within 5 min of cell dialysis, even in the absence of cyanide. Intracellular Mg<jats:sup>2+<\/jats:sup> ions played protective role at all temperatures. Decline of I<jats:sub>K1,tail<\/jats:sub> was accompanied by characteristic changes in its kinetics, as well as by changes in the kinetics of I<jats:sub>K1<\/jats:sub> inactivation, a marker of depletion of t-tubular K<jats:sup>+<\/jats:sup>. The data point to remodeling of t tubules as the primary reason for the observed effects. Consistent with this, detubulation of myocytes using formamide-induced osmotic stress significantly reduced I<jats:sub>K1,tail<\/jats:sub>, as well as the inactivation of inward I<jats:sub>K1<\/jats:sub>. Overall, the data provide strong evidence that changes in t tubule volume\/structure may occur on a short time scale in response to various types of stress. <\/jats:p>","DOI":"10.1152\/ajpheart.00304.2011","type":"journal-article","created":{"date-parts":[[2011,9,3]],"date-time":"2011-09-03T02:11:11Z","timestamp":1315015871000},"page":"H1984-H1995","source":"Crossref","is-referenced-by-count":14,"title":["Metabolic stress in isolated mouse ventricular myocytes leads to remodeling of t tubules"],"prefix":"10.1152","volume":"301","author":[{"given":"Lu-Feng","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuzhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anatoli N.","family":"Lopatin","sequence":"additional","affiliation":[{"name":"Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1768-322X.1995.tb00941.x"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.269.3.H1165"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00347.2002"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00005.2007"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000135547.53927.F6"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2008.12.3896"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1007\/BF00724526"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.012708"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0301i.x"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1007\/BF01516287"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.278.3.H886"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.557bd.x"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1007\/BF00656997"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.028175"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(00)00256-X"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.22.2586"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.277.2.H603"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-002-0851-8"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2000.1232"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.107227"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/503906"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2828(03)00014-2"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00002.2005"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2007.07.016"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.108.130617"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.3.266"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.95.271"},{"key":"B28","first-page":"11","volume":"6","author":"Takeshima H","year":"2000","journal-title":"Mol Cell"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.212324"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.113.5.661"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000030180.06028.23"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.83.8.806"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00304.2011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T22:06:55Z","timestamp":1567980415000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00304.2011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11]]},"references-count":32,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["10.1152\/ajpheart.00304.2011"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00304.2011","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11]]}}}