{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:17:08Z","timestamp":1792793828750,"version":"4.5.0"},"reference-count":73,"publisher":"American Physiological Society","issue":"11","funder":[{"name":"NIH NHLBI","award":["P01HL70687"],"award-info":[{"award-number":["P01HL70687"]}]},{"name":"NIH","award":["5T32GM927154"],"award-info":[{"award-number":["5T32GM927154"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2015,12,1]]},"abstract":"<jats:p>Perivascular adipose tissue (PVAT) reduces vasoconstriction to norepinephrine (NE). A mechanism by which PVAT could function to reduce vascular contraction is by decreasing the amount of NE to which the vessel is exposed. PVATs from male Sprague-Dawley rats were used to test the hypothesis that PVAT has a NE uptake mechanism. NE was detected by HPLC in mesenteric PVAT and isolated adipocytes. Uptake of NE (10 \u03bcM) in mesenteric PVAT was reduced by the NE transporter (NET) inhibitor nisoxetine (1 \u03bcM, 73.68 \u00b1 7.62%, all values reported as percentages of vehicle), the 5-hydroxytryptamine transporter (SERT) inhibitor citalopram (100 nM) with the organic cation transporter 3 (OCT3) inhibitor corticosterone (100 \u03bcM, 56.18 \u00b1 5.21%), and the NET inhibitor desipramine (10 \u03bcM) with corticosterone (100 \u03bcM, 61.18 \u00b1 6.82%). Aortic PVAT NE uptake was reduced by corticosterone (100 \u03bcM, 53.01 \u00b1 10.96%). Confocal imaging of mesenteric PVAT stained with 4-[4-(dimethylamino)-styrl]- N-methylpyridinium iodide (ASP<jats:sup>+<\/jats:sup>), a fluorescent substrate of cationic transporters, detected ASP<jats:sup>+<\/jats:sup>uptake into adipocytes. ASP<jats:sup>+<\/jats:sup>(2 \u03bcM) uptake was reduced by citalopram (100 nM, 66.68 \u00b1 6.43%), corticosterone (100 \u03bcM, 43.49 \u00b1 10.17%), nisoxetine (100 nM, 84.12 \u00b1 4.24%), citalopram with corticosterone (100 nM and 100 \u03bcM, respectively, 35.75 \u00b1 4.21%), and desipramine with corticosterone (10 and 100 \u03bcM, respectively, 50.47 \u00b1 5.78%). NET protein was not detected in mesenteric PVAT adipocytes. Expression of Slc22a3 (OCT3 gene) mRNA and protein in PVAT adipocytes was detected by RT-PCR and immunocytochemistry, respectively. These end points support the presence of a transporter-mediated NE uptake system within PVAT with a potential mediator being OCT3.<\/jats:p>","DOI":"10.1152\/ajpheart.00308.2015","type":"journal-article","created":{"date-parts":[[2015,10,3]],"date-time":"2015-10-03T00:23:26Z","timestamp":1443831806000},"page":"H1904-H1914","source":"Crossref","is-referenced-by-count":52,"title":["Organic cation transporter 3 contributes to norepinephrine uptake into perivascular adipose tissue"],"prefix":"10.1152","volume":"309","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8000-3997","authenticated-orcid":false,"given":"Nadia","family":"Ayala-Lopez","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William F.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Burnett","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James N.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Miami, Coral Gables, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice M.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie W.","family":"Watts","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-004-1346-2"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3109\/07853890.2012.663928"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1002\/prp2.41"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0800466105"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-008-0685-6"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00013.2005"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707229"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.114.303029"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(02)00435-6"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2014.02.005"},{"key":"B13","first-page":"103","volume":"60","author":"Centers for Disease Control and Prevention","year":"2011","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"B14","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.5858\/2007-131-1766a-PATTAE","volume":"131","author":"Chaldakov GN","year":"2007","journal-title":"Arch Pathol Lab Med"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1097\/FPC.0b013e32833fe789"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.301.3.1097"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2008.10.004"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1080\/09687680110033792"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1987.253.6.C783"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00656.2003"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/402053"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2007.05.025"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000220381.40739.dd"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707228"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.03.013"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.821181"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.60.52"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1177\/1087057107299524"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2011.05579.x"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-411512-5.00009-9"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(00)01408-7"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.01899.x"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-789X.2009.00623.x"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(90)90105-Q"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.103.053298"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.5049\/EBP.2013.11.2.46"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.01793.x"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0b013e328363ffaf"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-007-9254-z"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1038\/ajh.2010.172"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1007\/BF03013235"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60655-8"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2012.09.026"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.91415.2007"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0024com"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.2337\/db11-1603"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-015-3606-8"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2013.10.021"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(94)90251-8"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpsychires.2007.07.009"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00552.2013"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2014.732"},{"key":"B54","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1016\/S0022-3565(24)37161-7","volume":"283","author":"Owens MJ","year":"1997","journal-title":"J Pharmacol Exp Ther"},{"key":"B55","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1016\/S0022-3565(24)34961-4","volume":"290","author":"Paczkowski FA","year":"1999","journal-title":"J Pharmacol Exp Ther"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1097\/00000539-200211000-00032"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(99)00270-1"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1159\/000075316"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2396(20010101)39:1<32::AID-SYN5>3.0.CO;2-3"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1021\/mp400024d"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209824200"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(98)00119-0"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2011.04.012"},{"key":"B64","first-page":"277","volume":"13","author":"Soltis EE","year":"1991","journal-title":"Clin Exp Hypertens A"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1111\/j.1559-4564.2006.05510.x"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1326.2011.01378.x"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.2147\/VHRM.S33760"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1042\/CS20110151"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.06.001"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1998.5639"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000140058.28994.ec"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.113.301476"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1039\/c2ob26633d"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)66585-3"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2012.01.008"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00308.2015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T22:19:47Z","timestamp":1748643587000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00308.2015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,1]]},"references-count":73,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2015,12,1]]}},"alternative-id":["10.1152\/ajpheart.00308.2015"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00308.2015","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,1]]}}}