{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T15:00:27Z","timestamp":1772550027881,"version":"3.50.1"},"reference-count":26,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:p> Endothelium-denuded bovine pulmonary arteries (BPA) contract to hypoxia through a mechanism potentially involving removing a superoxide-derived hydrogen peroxide-mediated relaxation. BPA organ cultured for 24 h with 0.1 mM cobalt chloride (CoCl<jats:sub>2<\/jats:sub>) to increase the expression and activity of heme oxygenase-1 (HO-1) is accompanied by a decrease in 5 \u03bcM lucigenin-detectable superoxide and an increase in horseradish peroxidase-luminol detectable peroxide levels. Force development to KCl in BPA was not affected by increases in HO-1, but the hypoxic pulmonary vasoconstriction (HPV) response was decreased. Organ culture with a HO-1 inhibitor (10 \u03bcM chromium mesoporphyrin) reversed the effects of HO-1 on HPV and peroxide. Treatment of HO-1-induced BPA with extracellular catalase resulted in reversal of the attenuation of HPV without affecting the force development to KCl. Increasing intracellular peroxide scavenging with 0.1 mM ebselen increased force development to KCl and partially reversed the decrease in HPV seen on induction of HO-1. HO-1 induction increases extracellular (ec) superoxide dismutase (SOD) expression without changing Cu,Zn-SOD and Mn-SOD levels. HO-1-induced BPA rings treated with the copper chelator 10 mM diethyldithiocarbamate to inactivate ecSOD and Cu,Zn-SOD showed increased superoxide and decreased peroxide to levels equal to non-HO-1-induced rings, whereas the addition of SOD to freshly isolated BPA rings attenuated HPV similar to HO-1 induction with CoCl<jats:sub>2<\/jats:sub>. Therefore, HO-1 induction in BPA increases ecSOD expression associated with enhanced generation of peroxide in amounts that may not be adequately removed during hypoxia, leading to an attenuation of HPV. <\/jats:p>","DOI":"10.1152\/ajpheart.00315.2009","type":"journal-article","created":{"date-parts":[[2009,8,8]],"date-time":"2009-08-08T01:54:52Z","timestamp":1249696492000},"page":"H1453-H1461","source":"Crossref","is-referenced-by-count":24,"title":["Heme oxygenase-1 induction modulates hypoxic pulmonary vasoconstriction through upregulation of ecSOD"],"prefix":"10.1152","volume":"297","author":[{"given":"Mansoor","family":"Ahmad","sequence":"first","affiliation":[{"name":"Departments of 1Physiology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangmin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Departments of 1Physiology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa R.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Departments of 1Physiology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharath","family":"Kandhi","sequence":"additional","affiliation":[{"name":"Departments of 1Physiology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar","family":"Perez","sequence":"additional","affiliation":[{"name":"Departments of 1Physiology and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nader G.","family":"Abraham","sequence":"additional","affiliation":[{"name":"Pharmacology, New York Medical College, Valhalla, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael S.","family":"Wolin","sequence":"additional","affiliation":[{"name":"Departments of 1Physiology and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.107.07104"},{"key":"B2","first-page":"1464","volume":"28","author":"Abraham NG","year":"1987","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B3","first-page":"127","volume":"11","author":"Archer SL","year":"1986","journal-title":"Herz"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1987.252.4.H721"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1989.66.1.167"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1990.258.5.H1267"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00385.2001"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1990.259.4.H1056"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.86.12.1224"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2006-0331TR"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00629.2004"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.104.517102"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000024689.07590.C2"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00846.2007"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.276.1.H235"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1007\/0-306-46825-5_23"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1994.267.6.L823"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.21138"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00024.2005"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000036751.04896.F1"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1126\/science.1105010"},{"key":"B23","first-page":"245","volume-title":"Nitric Oxide and Radicals in the Pulmonary Vasculature","author":"Wolin MS","year":"1996"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00678.2002"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00632.2006"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206452200"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00315.2009","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T21:33:09Z","timestamp":1567978389000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00315.2009"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["10.1152\/ajpheart.00315.2009"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00315.2009","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,10]]}}}