{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:21:50Z","timestamp":1790734910396,"version":"4.1.0"},"reference-count":45,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"published-print":{"date-parts":[[2012,9,1]]},"abstract":"<jats:p> Apelin is an endogenous ligand for the angiotensin-like 1 receptor (APJ) and has beneficial effects against myocardial ischemia-reperfusion injury. Little is known about the role of apelin in the homing of vascular progenitor cells (PCs) and cardiac functional recovery postmyocardial infarction (post-MI). The present study investigated whether apelin affects PC homing to the infarcted myocardium, thereby mediating repair and functional recovery post-MI. Mice were infarcted by coronary artery ligation, and apelin-13 (1 mg\u00b7kg<jats:sup>\u22121<\/jats:sup>\u00b7day<jats:sup>\u22121<\/jats:sup>) was injected for 3 days before MI and for 14 days post-MI. Homing of vascular PCs [CD133<jats:sup>+<\/jats:sup>\/c-Kit<jats:sup>+<\/jats:sup>\/Sca1<jats:sup>+<\/jats:sup>, CD133<jats:sup>+<\/jats:sup>\/stromal cell-derived factor (SDF)-1\u03b1<jats:sup>+<\/jats:sup>, and CD133<jats:sup>+<\/jats:sup>\/CXC chemokine receptor (CXCR)-4<jats:sup>+<\/jats:sup>] into the ischemic area was examined. Myocardial Akt, endothelial nitric oxide synthase (eNOS), VEGF, jagged1, notch3, SDF-1\u03b1, and CXCR-4 expression were assessed at 24 h and 14 days post-MI. Functional analyses were performed on day 14 post-MI. Mice that received apelin-13 treatment demonstrated upregulation of SDF-1\u03b1\/CXCR-4 expression and dramatically increased the number of CD133<jats:sup>+<\/jats:sup>\/c-Kit<jats:sup>+<\/jats:sup>\/Sca1<jats:sup>+<\/jats:sup>, CD133<jats:sup>+<\/jats:sup>\/SDF-1\u03b1<jats:sup>+<\/jats:sup>, and c-Kit<jats:sup>+<\/jats:sup>\/CXCR-4<jats:sup>+<\/jats:sup> cells in infarcted hearts. Apelin-13 also significantly increased Akt and eNOS phosphorylation and upregulated VEGF, jagged1, and notch3 expression in ischemic hearts. This was accompanied by a significant reduction of myocardial apoptosis. Furthermore, treatment with apelin-13 promoted myocardial angiogenesis and attenuated cardiac fibrosis and hypertrophy together with a significant improvement of cardiac function at 14 days post-MI. Apelin-13 increases angiogenesis and improves cardiac repair post-MI by a mechanism involving the upregulation of SDF-1\u03b1\/CXCR-4 and homing of vascular PCs. <\/jats:p>","DOI":"10.1152\/ajpheart.00366.2012","type":"journal-article","created":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:15:26Z","timestamp":1341101726000},"page":"H605-H618","source":"Crossref","is-referenced-by-count":107,"title":["Apelin-13 increases myocardial progenitor cells and improves repair postmyocardial infarction"],"prefix":"10.1152","volume":"303","author":[{"given":"Lanfang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian-Xiong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709867105"},{"key":"B1a","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14232-8"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.192138"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000095788.78169.AF"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.2337\/db08-0503"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.04.452"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00236.2008"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.201186"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(07)70028-5"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1101\/gad.308904"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)01424-4"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.930404"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3727\/096368909X474843"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.3727\/096368910X504450"},{"key":"B14","first-page":"1986","volume":"119","author":"High FA","year":"2009","journal-title":"J Clin Invest"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.672451"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.06.028"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-008-9167-7"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1172\/JCI6889"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3434"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2007.12.015"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39733"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.03.004"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601982"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2007.10.002"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2003.11.002"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0740034.x"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1097\/01.md.0000085054.63483.40"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90495-O"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1097\/CRD.0b013e3181b3fe0d"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01177.2010"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2001.1516"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2004.08.014"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.01.012"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-007-0671-2"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1002\/9780470319413.ch7"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000033522.37861.69"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023114"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9489"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01250.2007"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvm108"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2008.02011.x"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035905"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2009.02.010"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.01.011"}],"container-title":["American Journal of Physiology-Heart and Circulatory Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.physiology.org\/doi\/pdf\/10.1152\/ajpheart.00366.2012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T21:33:15Z","timestamp":1567978395000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.physiology.org\/doi\/10.1152\/ajpheart.00366.2012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,1]]},"references-count":45,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,9,1]]}},"alternative-id":["10.1152\/ajpheart.00366.2012"],"URL":"https:\/\/doi.org\/10.1152\/ajpheart.00366.2012","relation":{},"ISSN":["0363-6135","1522-1539"],"issn-type":[{"value":"0363-6135","type":"print"},{"value":"1522-1539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,1]]}}}