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Exposure to 1 and 10 nM estradiol for 24 h reduced F<jats:sub>2\u03b1<\/jats:sub>-isoprostane production by 36 and 49%, respectively ( P &lt; 0.001 vs. control). Exposure to antiestrogens alone (ICI-182780 or EM-652) slightly reduced F<jats:sub>2\u03b1<\/jats:sub>-isoprostanes ( P &lt; 0.05 vs. control), but much less than exposure to estradiol ( P &lt; 0.05). ICI-182780 reversed the estradiol-induced reduction of F<jats:sub>2\u03b1<\/jats:sub>-isoprostane concentration ( P &lt; 0.05). Along with time-course analysis, these results suggest that estradiol effects were mediated through estrogen receptor-dependent and -independent mechanisms. Progestogens alone (progesterone or medroxyprogesterone acetate) did not modify F<jats:sub>2\u03b1<\/jats:sub>-isoprostane production at any of the tested concentrations (1, 10, and 100 nM). Progesterone completely reversed estradiol-induced reduction of F<jats:sub>2\u03b1<\/jats:sub>-isoprostane production ( P &lt; 0.05 vs. control and estradiol), but medroxyprogesterone acetate did not ( P &lt; 0.05 vs. control).<\/jats:p>","DOI":"10.1152\/ajpheart.00369.2002","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:04:39Z","timestamp":1425413079000},"page":"H2644-H2649","source":"Crossref","is-referenced-by-count":10,"title":["Estradiol reduces F<sub>2\u03b1<\/sub>-isoprostane production in cultured human endothelial cells"],"prefix":"10.1152","volume":"283","author":[{"given":"Carlos","family":"Hermenegildo","sequence":"first","affiliation":[{"name":"Research Unit, Hospital Clinic Universitari de Valencia and Departments of"},{"name":"Physiology,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u0131\u0301a 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